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BRAF突变分析:对8365个诊断样本进行回顾性评估,对犬种进行特别观察 (2018-2024年)

Marielle Appenzeller¹, Alexandra Kehl^1,2, Katrin Törner¹

¹LABOKLIN GmbH & Co. KG, 97688 Bad Kissingen, Germany.

Veterinary sciences

|August 28, 2025

概括

在狗中进行常规的BRAF V595E突变分析有助于诊断尿道癌 (UC). 特定品种的突变概率增加,表明从5岁开始进行特定品种的查.

科学领域:

兽医瘤学
狗病理学
分子诊断

背景情况:

这种BRAF V595E突变是狗尿癌 (UC) 的关键标志物.
尽管它用于诊断,但对狗BRAF突变的流行情况缺乏全面的数据分析.
了解人口和特定品种的分布对于有针对性的诊断至关重要.

研究的目的:

分析BRAF V595E突变检测的狗样本中的年龄,性别和品种分布.
为了确定携带与UC相关的BRAF突变的可能性较高的品种.
建立潜在的年龄建议,以对易受感染的品种进行查.

主要方法:

在2018年至2024年间对8365只狗诊断样本 (尿液,细胞学,组织学) 的分析.
使用滴状数字PCR (ddPCR) 检测BRAF V595E突变
统计分析包括逻辑回归模型 (odds比) 来评估特定品种的概率.

主要成果:

一些品种,包括苏格兰牧羊犬和夏特兰牧羊犬,显著增加了BRAF突变的几率.
在易受感染的品种中,最年轻的BRAF阳性狗在5岁时被发现.
数据提供了首次公布的BRAF突变在大型犬类诊断队列中的流行率分析.

结论:

在狗UC中明显存在对BRAF V595E突变的品种倾向.
在危险品种中,从5岁开始对BRAF突变进行查可能是有益的.
这项研究为完善犬尿癌的诊断和查策略提供了必要的数据.

关键词:

BRAF V595E 突变一只狗标志使用过渡性细胞癌泌尿器癌

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