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在生产性复制过程中,小鼠细胞巨病毒和人类细胞巨病毒在不同细胞类型的炎症诱导上有所不同

Jessica J Carter¹, Daniel H Schneider¹, Arshaan M Hisamuddin¹

¹Viral Immunology Center, Department of Biology, Georgia State University, Atlanta, GA 30303, USA.

|August 28, 2025

概括

肌死是一种被编程的细胞死亡,由小鼠细胞中小鼠细胞巨变病毒 (MCMV) 诱导,而不是人类细胞中人类细胞巨变病毒 (HCMV) 诱导. 这表明热的诱导取决于宿主细胞,并且可能是特定于物种的.

科学领域:

免疫学
病毒学
细胞生物学

背景情况:

炎症性细胞死亡 (PCD) 是对宿主防御病毒感染至关重要的.
之前的研究表明,使用与艾滋病相关的人类细胞巨化病毒 (HCMV) 视网膜亡的小鼠模型,对小鼠细胞巨化病毒 (MCMV) 视网膜亡有影响.

研究的目的:

调查 MCMV 和 HCMV 诱导热细胞是否扩展到不同的小鼠和人类细胞类型.
确定宿主细胞类型和物种特异性在病毒感染期间诱导热的作用.

主要方法:

在体外研究,将感染MCMV的小鼠纤维细胞/巨细胞与感染HCMV的人类纤维细胞/ARPE-19细胞进行比较.
烧死标记的分析:卡斯巴-1,气体皮质G (GSDMD) 和介质蛋白 (IL) -18/IL-1β的转录.
使用紫外线无活化的MCMV进行研究,以评估体蛋白的作用.

主要成果:

在生产性复制过程中,MCMV显著刺激了小鼠纤维细胞和巨细胞中与烧死相关的转录.
在生产性复制过程中,HCMV没有显著刺激人体纤维细胞或ARPE-19细胞中的这些转录.
在小鼠纤维细胞中,MCMV的病毒结膜蛋白并没有涉及激光死诱导.

结论:

在MCMV和HCMV生产性复制过程中诱导热取决于宿主细胞类型.
这些发现表明,热诱导可能会在MCMV和HCMV之间表现出物种特异性.
必须考虑病毒编码细胞死亡 (PCD) 抑制剂在调节热的潜在作用.

关键词:

艾滋病一个女士抑制细胞死亡人类细胞巨核病毒鼠类细胞巨核病毒热症视网膜炎

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