Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

7.6K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
7.6K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Serendipita indica promotes rice phosphorus uptake by plasma membrane H+-ATPase OsA1-stimulated root hair growth.

Journal of experimental botany·2026
Same author

Broadly neutralizing antibodies against HIV-1 pseudoviruses elicited by envelope trimer DNA with chimeric design delivered <i>via</i> silica-calcium phosphate nanoparticles.

Nanoscale·2026
Same author

Systemic Immune-Inflammatory Index For Evaluating Robotic and Laparoscopic Proximal Gastrectomy in Upper Gastric Cancer.

Journal of visualized experiments : JoVE·2026
Same author

Biodegradable plastic mulches: Distinct effects on microbial communities but no impact on soil multifunctionality during cabbage production.

Journal of hazardous materials·2026
Same author

Discharge health education needs and experiences of patients with skeletal-related events from bone metastases: A qualitative study.

PloS one·2026
Same author

Metal Node Engineering by Decorating Ferrocene Complexes To Enhance the CO<sub>2</sub> Photoreduction Performance of Metal Organic Frameworks.

Inorganic chemistry·2026
Same journal

Gas-Responsive Metal-Organic Frameworks for Adaptive Thermal Energy Storage with Tunable Charge-Discharge Temperatures.

Journal of the American Chemical Society·2026
Same journal

Engineering a Thiamine-Dependent Benzoylformate Decarboxylase for Stereodivergent Radical C(sp<sup>3</sup>)-C(sp<sup>3</sup>) Bond Formation.

Journal of the American Chemical Society·2026
Same journal

Accelerated Directional Proton-Coupled Electron Transfer Enabled by Intrinsic Dipole Field in Biomimetic α-Helical Structure.

Journal of the American Chemical Society·2026
Same journal

Alternating Current-Driven Hydrogen Isotope Labeling of Aliphatic Amines Using 1,3-Propanedithiol as an Efficient Hydrogen Atom Transfer Reagent.

Journal of the American Chemical Society·2026
Same journal

Two-Dimensional van der Waals Polar Metal MoOBr<sub>2</sub>.

Journal of the American Chemical Society·2026
Same journal

Negatively Curved Chiral Bilayer Nanographene.

Journal of the American Chemical Society·2026
查看所有相关文章

相关实验视频

Updated: Sep 9, 2025

Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

9.9K

通过系统间交叉抑制增强单分子成像

Fan Ding1, Song Chen1, Fei Du1

  • 1Department of Chemistry and Shanghai Key Laboratory of Molecular Catalysis and Innovative Materials, Fudan University, Shanghai 200438, China.

Journal of the American Chemical Society
|August 28, 2025
PubMed
概括
此摘要是机器生成的。

研究人员开发了一种新的Cy5染料衍生物Cy5-N,可显著提高单分子成像的光稳定性和信号噪声比. 这种创新克服了传统Cy5染料的局限性,使生物样本的观察更清晰,更长时间.

更多相关视频

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion STED Nanoscopy
10:00

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion STED Nanoscopy

Published on: March 24, 2014

78.2K
Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
20:00

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Published on: October 31, 2015

14.0K

相关实验视频

Last Updated: Sep 9, 2025

Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

9.9K
Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion STED Nanoscopy
10:00

Visualization of the Immunological Synapse by Dual Color Time-gated Stimulated Emission Depletion STED Nanoscopy

Published on: March 24, 2014

78.2K
Single Molecule Fluorescence Microscopy on Planar Supported Bilayers
20:00

Single Molecule Fluorescence Microscopy on Planar Supported Bilayers

Published on: October 31, 2015

14.0K

科学领域:

  • 化学生物学
  • 光谱学
  • 生物物理

背景情况:

  • 色素5 (Cy5) 对于单分子成像至关重要,但由于三重状态的形成而受到光漂白和信号间歇性的影响.
  • Cy5的三重状态促进了反应性氧物种 (ROS) 的产生,易受核友攻击,限制了其在生物系统中的使用.

研究的目的:

  • 设计新型替代Cy5衍生物,抑制系统间交叉 (ISC) 并减少三重状态群体.
  • 提高Cy5的光稳定性,减少间歇性,并改善高级生物成像应用的信号噪声比 (SNR).

主要方法:

  • 计算化学用于理论计算.
  • 暂时吸收光谱验证ISC抑制.
  • 包括Cy5-N在内的替代Cy5衍生物的合成和表征
  • 在体外和活体U2OS细胞中进行单分子成像实验.

主要成果:

  • 工程制造的Cy5衍生品显示ISC被抑制.
  • 与标准的Cy5相比,化合物Cy5-N的光稳定性增加了790%,SNR提高了2.3倍.
  • Cy5-N 呈现连续的排放和对核友性攻击的抵抗性 (例如来自β-乙醇).
  • 在活细胞中使用Cy5-N进行长期单分子追踪,并改善了SNR.

结论:

  • 一种新的分子设计策略有效地提高了单分子成像的染剂性能.
  • Cy5-N提供了卓越的光稳定性和SNR,克服了传统Cy5的主要局限性.
  • 这种进步具有改善生物成像和传感技术的巨大潜力.