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[人工增强促进体TCIN5B的构建和优化]

Meng Guo¹, Shuxin Dong¹, Jun Li¹

¹Key Laboratory of Medicinal Molecule Science and Pharmaceutical Engineering, Ministry of Industry and Information Technology, Institute of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 100081, China.

Sheng wu gong cheng xue bao = Chinese journal of biotechnology

|August 28, 2025

概括

研究人员为酵母细胞工厂开发了新的应激反应促进剂. 这种策略在高温压力条件下增强了天然化合物的生产.

科学领域:

合成生物学
分子生物学

背景情况:

工业酵母菌株需要强大的促进剂来产生稳定的化合物.
高温压力对细胞功能和产品产量产生负面影响.
了解转录因子-DNA相互作用对于促进体工程至关重要.

研究的目的:

为了在酵母中增强基因表达,
开发基于转录因子结合点的人造促进剂的框架.
在压力条件下提高酵母中天然化合物生产的稳定性和产量.

主要方法:

挖掘转录组数据以确定应激反应的转录因子 (例如,CIN5).
描述转录因子结合基因 (例如,CIN5BS) 的调节活性.
通过将cis元素插入现有的促进器区域 (例如TEF1) 来设计人工促进器.
优化 cis 元素以增强 G+C 含量以提高促进体强度.

主要成果:

鉴定了转录因子CIN5及其结合基因CIN5BS,具有转录增强活性.
构建了四个人工促进剂 (TCIN5B3-6) 实现高达15.25倍的转录增强.
设计了五个优化的cis元素 (CIN5B4-1-CIN5B4-5),导致更强的促进剂.
在37°C时表现出一种促进剂 (TCIN5B4-1),活性比对照剂高4. 71倍.

结论:

通过转录因子挖掘和cis元素工程来设计应激响应促进体的技术框架.
开发了酵母细胞工厂的战略,以提高高温压力下的天然化合物的生产.
为工业生物技术应用设计强大的酵母菌株提供了宝贵的参考资料.

关键词:

类植物发起人工程应激反应转录因子 CIN5

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