通过对抗性域适应来解决光学和生物变异性的食物传播细菌分类的AI显微镜的改进
Siddhartha Bhattacharya1,2, Aarham Wasit1,2, J Mason Earles3,4
1Department of Biosystems and Agricultural Engineering, Michigan State University, East Lansing, MI, United States.
Frontiers in artificial intelligence
|August 28, 2025
概括
域适应技术增强了细菌分类的AI显微镜,在各种条件下提高了准确性. 这种方法使得人工智能显微镜在资源有限的环境中更可靠.
科学领域:
- 微生物学
- 计算机科学
- 生物技术
背景情况:
- 支持人工智能的显微镜提供快速的细菌分类,但在动态或资源有限的环境中难以进行成像变化.
- 现有的人工智能模型在应用于不同显微镜设置或条件的数据时往往缺乏通用性.
研究的目的:
- 使用域适应技术增强人工智能驱动的细菌分类的通用性和稳定性.
- 开发适用于分散和资源有限的AI显微镜的可扩展框架.
主要方法:
- 使用域对抗神经网络 (DANNs) 和多DANNs (MDANNs) 来处理单个和多个域的变化.
- 在数据有限的场景中使用了EfficientNetV2骨干,用于细粒度的特征提取和可扩展性.
- 在最佳条件下对六种细菌的源域进行训练,并在使用最小标记数据的不同模式,放大和化时间的目标域进行测试.
主要成果:
- DANNs在目标域的细菌分类精度显著提高,高达54.5% (例如20倍放大:34.4%至88.9%),源域的性能损失最小.
- 在明亮场域 (BF) 中,MDANNs的精度从73.3%提高到76.7%.
- 特征可视化技术 (Grad-CAM,t-SNE) 证实了模型学习域不变特征的能力.
结论:
- 域适应技术有效地提高了AI显微镜在各种成像条件下的细菌分类的普遍性.
- 开发的框架为细菌识别提供了可扩展和适应的解决方案,在具有挑战性的现实环境中扩大了AI显微镜的实用性.
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