用于超敏感和选择性检测可生物利用Hg的全细胞生物传感器
Jiangpeng Tang1,2, Chang-Ye Hui2, Shunyu Hu1,2
1Department of Toxicology, School of Public Health, Southern Medical University Guangzhou 510515 China huangzhenlie858252@smu.edu.cn yanguo615@163.com.
RSC advances
|August 28, 2025
概括
我们开发了一种全细胞生物传感器, 这种肉眼感应器使用皮欧梅拉宁生产来快速,低成本和选择性地监测环境.
科学领域:
- 环境科学
- 微生物学
- 生物技术
背景情况:
- 在环境水中检测微量无机 (Hg) 对公众健康至关重要.
- 现有的Hg检测方法可能是昂贵的,缓慢的,或需要专门的设备.
- 需要快速,低成本和现场部署的Hg2传感器.
研究的目的:
- 设计一个全细胞生物传感器,以快速,低成本和肉眼检测环境水中的Hg.
- 作为染色体报告系统,利用黑素生物合成途径.
- 在灵敏度,选择性和稳定性方面评估工程生物传感器的性能.
主要方法:
- 通过将MerR-Pmer元素合到pyomelanin路径来进行工程化Escherichia coli.
- 编码优化并选了三种4-基酸二氧化酶 (HppD) 同类物.
- 在培养上游物中通过400nm的吸收量测量皮梅拉宁的产生.
主要成果:
- TOP10/pHg-PAO1传感器菌株显示出一个广的定量范围 (4.9-1250 nM) 和一个低的检测极限 (1.2 nM).
- 允许在至少14小时内进行无设备的直接测量,并保持色素稳定性.
- 生物传感器显示出对其他测试离子的绝对选择性,并在各种水矩阵中保持准确性.
结论:
- 工程化大肠杆菌作为一个强大的,用户友好的Hg全细胞生物传感器.
- 黑素是下一代全细胞传感器的可行的染色记者.
- 开发的生物传感器可以在现场无需设备对环境水域的污染进行查.
相关概念视频
01:15High-Performance Liquid Chromatography: Types of Detectors
2.3K
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
2.3K
01:17Microbial Biosensors
88
Microbial biosensors are analytical devices that utilize living microbes to detect specific substances through measurable signals. These devices consist of two main components: biosensing organisms and signal-transducing elements. Biosensing organisms, such as Escherichia coli or Saccharomyces cerevisiae, are typically housed in multiwell plates connected to transducers, enabling rapid, real-time detection of target analytes.Signal Generation MechanismWhen a target analyte—such as...
88