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相关概念视频

Gene Evolution - Fast or Slow?02:05

Gene Evolution - Fast or Slow?

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The genomes of eukaryotes are punctuated by long stretches of sequence which do not code for proteins or RNAs. Although some of these regions do contain crucial regulatory sequences, the vast majority of this DNA serves no known function. Typically, these regions of the genome are the ones in which the fastest change, in evolutionary terms, is observed, because there is typically little to no selection pressure acting on these regions to preserve their sequences.
In contrast, regions which code...
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The Eukaryotic Promoter Region02:40

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Gene Duplication and Divergence02:37

Gene Duplication and Divergence

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The seminal work of Ohno in 1970 popularized the idea of gene duplication and divergence. DNA sequence comparison studies reveal that a large portion of the genes in bacteria, archaebacteria, and eukaryotes was  generated by gene duplication and divergence, indicating its critical role in evolution.
The duplicated copies of the gene are called Paralogs. Paralogs with similar sequences and functions form a gene family. Across several species, a large number of gene families are...
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Gene Conversion02:08

Gene Conversion

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Other than maintaining genome stability via DNA repair, homologous recombination plays an important role in diversifying the genome. In fact, the recombination of sequences forms the molecular basis of genomic evolution. Random and non-random permutations of genomic sequences create a library of new amalgamated sequences. These newly formed genomes can determine the fitness and survival of cells. In bacteria, homologous and non-homologous types of recombination lead to the evolution of new...
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Combinatorial Gene Control02:33

Combinatorial Gene Control

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Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
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Conservative Site-specific Recombination and Phase Variation02:53

Conservative Site-specific Recombination and Phase Variation

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Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
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Updated: Sep 9, 2025

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression
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基于深度生成和动态进化算法的新发起者设计方法

Yijun Gu1, Jianye Su2, Junfeng Xia2

  • 1Information Materials and Intelligent Sensing Laboratory of Anhui Province, Anhui University, 111 Jiulong Road, Hefei 230601 Anhui, China.

Nucleic acids research
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概括
此摘要是机器生成的。

我们开发了PromoDGDE,一种新方法来设计具有可调节基因表达水平的合成促进剂. 这种方法通过为*大肠杆菌*和*小肠杆菌*创造功能性促进剂来增强代谢工程和基因疗法.

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科学领域:

  • 合成生物学
  • 计算生物学
  • 分子生物学

背景情况:

  • 促进剂对于控制基因表达至关重要,对于代谢工程和基因治疗至关重要.
  • 目前的推广器设计方法往往优先考虑高表达,而忽视了可调节的调控强度的需要.

研究的目的:

  • 推出一种新的深度学习框架,用于设计具有特定表达水平的合成促进剂.
  • 证明该方法对大肠杆菌和大肠杆菌的有效性.

主要方法:

  • 使用扩散GAN学习自然促进物序列特征并产生新型候选物.
  • 使用强化学习和进化算法来动态优化合成促进子序列.

主要成果:

  • PromoDGDE在中表现优于现有的方法,产生具有理想功能的生物相关促进剂.
  • 在体内实验证实了合成促进剂的表达活性,超过60%的人获得了预期的调节作用.

结论:

  • 通过精确的监管,PromoDGDE为设计合成促进剂提供了有效和灵活的解决方案.
  • 该方法对合成生物学,包括代谢途径构建和基因疗法的应用具有重要意义.