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Updated: Sep 9, 2025

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
10:01

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Published on: November 28, 2017

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通过交联质谱和机器学习绘制Toxoplasma gondii互动体

Tadakimi Tomita1, Elizabeth Weyer1, Rebekah B Guevara1

  • 1Department of Pathology, Albert Einstein College of Medicine, New York, New York, USA.

mBio
|August 28, 2025
PubMed
概括

这项研究使用先进的交联质谱 (XL-MS) 和机器学习来绘制Toxoplasma gondii寄生虫中的蛋白相互作用. 这些发现揭示了这种广泛传播的人类病原体的新分子细节和潜在治疗点.

关键词:
一个GRA毒素菌在XL-MS互动体机器学习质谱学蛋白质交联剂蛋白与蛋白的相互作用蛋白质组学核糖体

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Identification of MyoD Interactome Using Tandem Affinity Purification Coupled to Mass Spectrometry14:47

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Protein Networks02:26

Protein Networks

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Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
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Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
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