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通过调节CsRAP2.3-CsERF1级联,CsLOB1-CsERF027调节模块积极增强果点疾病的抵抗力

Fengjiao Liu¹, Siyu Long¹, Junhua Hu¹

¹Citrus Research Institute, Southwest University, Chongqing, China.

Plant biotechnology journal

|August 28, 2025

概括

类植物对点病的抗性涉及到CsLOB1基因,该基因激活了防御途径. 通过调节关键基因和维持细胞平衡,过度表达CsLOB1可以增强抗病能力.

科学领域:

植物病理学
分子生物学
植物与微生物的相互作用

背景情况:

由Pseudofabraea citricarpa引起的果病对全球果产量产生重大影响.
果对这种病原体耐药性的分子基础尚不清楚.
识别参与果防御的关键基因和途径对于开发可持续的疾病管理策略至关重要.

研究的目的:

确定和描述LBD家族基因CsLOB1在类植物中对P. citricarpa耐药性的作用.
阐明CsLOB1调解的防御反应的分子机制.
研究涉及CsLOB1介导抗性的信号通路.

主要方法:

在不同果品种和病原体注射后对CsLOB1的基因表达分析.
产生和分析过度表达 (OE-CsLOB1) 或沉默 (CsLOB1-RNAi) 的转基因.
分子测定包括酵母单杂交,酵母双杂交,双分子光补充 (BiFC) 和病毒诱导的基因沉默 (VIGS).
测量反应性氧物种 (ROS),过氧化 (H2O2) 和甲 (MDA) 的含量.

主要成果:

CsLOB1的表达是由P. citricarpa引起的,并且在耐药果品种中更高.
转基因OE-CsLOB1植物显示疾病易感性降低,增强防御基因表达 (CsPR1,CsPR2,CsPR4),并保持ROS平衡.
CsLOB1 直接与 CsERF027 相互作用,上调 CsRAP2.3,反过来激活 CsERF1 和 CsPDF1.2,这是依赖 JA/ ET 的 ERF/ ORA59 途径中的关键基因.
沉默CsRAP2. 3,CsERF1或CsERF027增加了疾病发病率,而它们的过度表达则产生了耐药性.

结论:

CsLOB1在调解类植物对点病的抵抗方面发挥着至关重要的作用.
CsLOB1通过与CsERF027形成转录复合体来激活CsRAP2.3-CsERF1调节级联,触发JA/ET依赖的ERF/ORA59信号通路.
这项研究揭示了类植物抗病的新型分子机制,强调了CsLOB1作为基因改进的潜在目标.

关键词:

形植物形植物在CsLOB1 JA/ET信号通道疾病耐药性

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