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在酵母细胞中识别转录组范围内的核糖体碰撞的Disome-seq协议

Pedro H Ayres-Galhardo¹, James Marks¹, Sezen Meydan²

¹Department of Biochemistry, Vanderbilt University, Nashville, TN 37232, USA.

|August 28, 2025

概括

当翻译延长停止时形成的双胞体或核糖体碰撞复合体. 这种新的Disome-seq协议允许研究人员在酵母细胞中检测整个转录组中的二位体.

科学领域:

分子生物学
遗传学
细胞生物学

背景情况:

停滞的核糖体可能导致核糖体碰撞,形成双核糖体.
双体是触发信号通路的细胞复合体.
了解体频率和分布对于细胞调节至关重要.

研究的目的:

提供Saccharomyces cerevisiae中Disome-seq的详细方案.
为了实现全转录组的检测和分析.

主要方法:

酵母细胞培养和生长.
核糖体碰撞复合物的分离.
序列库准备用于全转录组分析.

主要成果:

这项研究提出了Disome-seq的验证方案.
这种方法可以在酵母转录组中检测出异体.

结论:

迪索姆-seq协议有助于研究酵母体中的核糖体碰撞.
这种技术对于研究体形成的生物学作用和后果至关重要.

关键词:

细胞生物学基因表达基因组学模型生物分子生物学测序方式

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相关概念视频

RNA-seq

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...