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一种快速查方法,用于检测乳制品中的安比西林残留物

Nida Noreen¹, Bushra Akhtar², Chi-Chung Chou³

¹Institute of Physiology and Pharmacology, University of Agriculture, Faisalabad, Pakistan.

Research in veterinary science

|August 28, 2025

概括

一种新的侧流检测 (LFA) 可以快速检测牛奶中的安培残留物. 这种快速方法有助于确保整个供应链的抗生素污染.

科学领域:

兽医科学
食品安全问题
分析化学

背景情况:

在乳制品动物中使用抗生素有助于抗菌素耐药性.
对牛奶中的抗生素残留物进行监测对于公共卫生和食品安全至关重要.

研究的目的:

开发一种快速和灵敏的乳制品乳中的安比西林残留物检测方法.
在牛奶样本中创建一种查工具.

主要方法:

开发一种基抗原结合物 (羊素-牛血清白蛋白).
产生和净化抗素的多克隆抗体.
抗体与黄金纳米颗粒的结合用于侧向流量测试 (LFA) 条形发展.

主要成果:

已成功创建多层LFA带用于安培检测.
开发的LFA显示了安培残留的快速检测.
该试验在8-10分钟内检测到最大残留量 (MRL) 为5ppb的安皮西林.

结论:

开发的LFA提供了一种快速有效的方法来选牛奶中的安比西林残留物.
这种测试可以在整个牛奶供应链中实施,从生产到消费者.
这种方法有助于通过监测奶牛养殖中的抗生素使用来控制抗菌素耐药性.

关键词:

安皮西林残留物抗体黄金纳米粒子侧流检测牛奶

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