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在非生物压力下对Solanum nigrum的参考基因进行全面验证:识别用于除草剂耐药性的pendimethalin响应基因

Wen Chen¹, Yufei Zhao², Xu Yang²

¹College of Agriculture, Tarim University, Alaer 843300, China; Key Laboratory of Genetic Improvement and Efficient Production for Specialty, Crops in Arid Southern Xinjiang of Xinjiang Corps, Alaer 843300, China; Key Laboratory of Integrated Pest Management of Xinjiang Production and Construction Corps in Southern Xinjiang, Alaer 843300, China; State Key Laboratory of Cotton Bio-breeding and Integrated Utilization, Anyang, 455000, China.

Ecotoxicology and environmental safety

|August 28, 2025

概括

这项研究确定了在各种压力下使黑夜 (Solanum nigrum L.) 的基因表达正常化的最佳基因. 这些验证的基因对于理解杂草适应除草剂和其他环境挑战至关重要.

科学领域:

植物分子生物学
杂草科学
基因组学和转录组学

背景情况:

黑夜 (Solanum nigrum L.) 是一种适应性强的入侵性杂草.
可靠的基因表达研究需要验证的参考基因来实现RT-qPCR正常化.
对于S. nigrum在压力下存在的参考基因尚未确定.

研究的目的:

在六种非生物压力下系统评估S. nigrum中RT-qPCR正常化候选基因.
为了确定每个压力条件的最佳基因对.
使用已确定的参考基因验证涉及除草剂反应的候选基因.

主要方法:

通过RefFinder集成的四个算法 (Delta Ct,geNorm,NormFinder,BestKeeper) 对八个候选参考基因的评估.
在寒冷,干旱,盐度,热量,暴露和甲治疗下对基因表达的分析.
通过RNA测序 (RNA-seq) 检测与甲反应相关的基因,随后进行RT-qPCR验证.

主要成果:

确定S. nigrum的压力特异性最佳基因对.
最佳配对包括TUB/EF-2 (冷),ACT/TUA (干旱),GAPDH/TUA (盐),TUA/EIF4A (Cd),TUA/EF-2 (热),以及TUB/ACT (甲).
参与甲适应的候选基因 (转录因子,除草剂代谢酶,过氧化酶) 的验证.

结论:

这项研究为S. nigrum叶子的基因表达正常化提供了第一个标准化的框架.
已识别的参考基因可以可靠地验证转录基因数据.
这些发现有助于了解杂草适应除草剂的分子机制.

关键词:

没有生命的压力抗除草药性甲参考基因黑色

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