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植物延迟发光的高分辨率成像

Yan-Xia Liu¹, Hai-Yu Fan¹, Yu-Hao Wang²

¹Center for Quantum Technology Research, School of Physics, Beijing Institute of Technology, Beijing 100081, China.

Journal of photochemistry and photobiology. B, Biology

|August 28, 2025

概括

这项研究引入了一种高灵敏度延迟发光 (DL) 成像系统,用于植物表型. 该系统揭示了DL强度的空间变化和特定物种对压力的反应,有助于优化植物环境.

科学领域:

植物科学
光物理学
生物光子学

背景情况:

延迟发光 (DL) 通过时空动力学提供了对光物理机制的洞察.
对DL的定量分析对于理解分子放松状态至关重要.

研究的目的:

开发一个高灵敏度的延迟发光成像系统.
实现全场时空DL成像的兆像素分辨率.
建立植物研究中DL动态的理论框架.

主要方法:

使用具有单光子计数分辨率的定量科学互补金属氧化物半导体 (qCMOS) 摄像头.
优化了光学架构和信号处理算法.
开发了一种两级量子模型,将DL动态与激发状态电子群体联系起来.

主要成果:

在*Arabidopsis thaliana*中观察到DL强度的空间异质性,静脉和损伤部位的信号更高.
在*Hydrocotyle vulgaris*和*Ginkgo biloba*中检测到特定物种对氧化应激的DL反应.
发现白光最大化了DL强度,而红光和蓝光则不同调节了衰变动力.

结论:

开发的DL成像系统为压力下的植物表型提供了一个框架.
这项研究提升了对光物理研究和光环境优化的理论理解.
结果突显了DL在非侵入性植物应激评估方面的潜力.

关键词:

延迟发光时间照片激发状态的放松量子成像空间异质性在QCMOS

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