在垃圾填埋场发现转化和甲原体的共同演变
Xiaocui Xiao1, Yuqian Wang1, Feng Huang1
1College of Energy Environment and Safety Engineering, College of Carbon Metrology, China Jiliang University, Hangzhou 310018, China.
Journal of hazardous materials
|August 28, 2025
概括
垃圾填埋场中的甲基生物驱动了 (As) 脱甲基化,将有毒甲基化As (MeAs) 转化为无机As (iAs). 抑制甲素增加了MeAs,突出了它们在循环和排毒中的关键作用.
科学领域:
- 环境微生物学
- 地质化学
- 的生物地质化学
背景情况:
- 垃圾填埋场是 (As) 污染的主要来源,毒性取决于化学物种.
- 甲甲基化可以解毒甲,而甲基化可以重新调动它.
- 甲素是填埋有机物降解的关键,但它们在As转化中的作用尚未得到充分研究.
研究的目的:
- 在模拟的垃圾填埋区 (LSZ) 中研究甲介导的As转化.
- 阐明甲碳代谢与A循环之间的联系.
- 了解甲对物种化和脱甲基化的影响.
主要方法:
- 填埋区和条件的模拟
- 使用甲基抑制剂2-甲硫酸盐 (BES) 来评估甲基活性.
- 甲基化 (MeAs) 和无机 (iAs) 种类的量化.
- 对甲基因丰度 (mcrA,arsI) 和KEGG通路注释的分析.
主要成果:
- 甲素被确定为MeAs转化为iAs的主要驱动因素.
- 通过抑制脱甲基化,BES治疗显著增加了MeAs水平 (1. 19倍).
- 在BES处理的样本中观察到mcrA和arsI基因的丰度降低.
- 甲基化途径 (M00356) 与甲化相关.
结论:
- 甲基在垃圾填埋场中通过将MeAs脱甲基化为iAs起到关键作用.
- 抑制甲原体会影响的解毒,导致MeAs的积累增加.
- 了解甲与的相互作用对于管理垃圾填埋环境中的污染至关重要.
相关概念视频
Overview of Archaea
132
Archaea, named after the Archaean eon, represent a unique domain of life, distinct from bacteria and eukaryotes, with remarkable traits. Their cellular and molecular features, ecological adaptability, and industrial relevance highlight their importance in understanding life processes and leveraging biotechnology.Cellular and Molecular CharacteristicsA defining feature of archaea is their unique membrane composition. Archaeal membranes contain ether-linked isoprenoid lipids, which confer...
132
Metabolism of Chemolithotrophs
165
Chemolithotrophs are microorganisms that obtain energy by oxidizing inorganic molecules such as hydrogen gas (H₂), ammonia (NH₃), reduced sulfur compounds (H₂S, S²⁻), and ferrous iron (Fe²⁺). Unlike heterotrophic organisms that rely on organic carbon, chemolithotrophs transfer electrons from these inorganic donors to the electron transport chain (ETC), generating a proton motive force (PMF) that drives ATP synthesis through oxidative phosphorylation.
165
Microbial Nutrition
287
Organisms exhibit remarkable metabolic diversity, categorized based on how they acquire energy and carbon. These strategies enable survival in various ecological niches and are essential for maintaining energy flow and nutrient cycling within ecosystems.Energy and Carbon SourcesOrganisms are classified as phototrophs or chemotrophs based on energy acquisition. Phototrophs use light as their energy source, while chemotrophs rely on oxidizing chemical compounds. Further differentiation arises...
287
Diversity of Archaea I
94
Archaea, a domain of single-celled microorganisms, are classified into five major phyla based on genetic and biochemical characteristics: Euryarchaeota, Crenarchaeota, Thaumarchaeota, Korarchaeota, and Nanoarchaeota. Among these, the phylum Euryarchaeota is notable for its remarkable diversity in morphology, metabolism, and ecological adaptations.Morphological and Metabolic DiversityMembers of Euryarchaeota exhibit a variety of cellular shapes, including rods and cocci. Their metabolic pathways...
94
Diversity of Archaea III
71
Crenarchaeota, a prominent phylum of Archaea, is remarkable for its ability to thrive in extreme environments characterized by high temperatures and acidity. These microorganisms inhabit sulfuric hot springs, volcanic systems, and submarine hydrothermal vents, where temperatures often exceed 100°C. The unique adaptations of Crenarchaeota not only allow survival under such extreme conditions but also provide insights into the mechanisms of life in primordial Earth-like...
71
Environmental Applications of Microorganisms
222
Microorganisms play a pivotal role in maintaining ecosystem balance by recycling essential elements such as carbon, nitrogen, and phosphorus, as well as supporting processes like bioremediation, wastewater treatment, and biofuel production.Microbes in Elemental CyclesIn the carbon cycle, microorganisms decompose organic matter, releasing carbon dioxide via aerobic respiration. This carbon dioxide is subsequently used by photosynthetic organisms to synthesize organic compounds, closing the...
222


