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Updated: Sep 9, 2025

Streamlined Purification of Plasmid DNA From Prokaryotic Cultures
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水染色学净化线性化等离子体DNA 在体外转录过程中减少dSRNA的形成

Juan Martínez-Oliván1, Verónica Lampaya1, Ana Larraga1

  • 1Certest Pharma, RNA Synthesis and Development Department, Certest Biotec, Zaragoza, Spain.

Methods in molecular biology (Clifton, N.J.)
|August 28, 2025
PubMed
概括

在mRNA生产过程中,优化的DNA模板净化减少了双链RNA (dsRNA). 这种方法最大限度地减少了杂质,从而降低了dSRNA感应受体 (如Toll-like受体3) 触发的先天免疫反应.

关键词:
亲和色谱水色谱在线培训免疫性疫苗的使用dsRNA 的时间在 mRNA 中

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