皮肤转录组学揭示了人体内的微生物活动和基因表达的变化
Minghao Chia1,2, Amanda Hui Qi Ng3, Aarthi Ravikrishnan3
1Genome Institute of Singapore (GIS), Agency for Science, Technology and Research (A*STAR), Singapore, Republic of Singapore. chia_minghao@a-star.edu.sg.
Nature biotechnology
|August 28, 2025
概括
我们开发了一种新的皮肤转录学方法来研究皮肤微生物. 这种方法揭示了微生物活动和相互作用,突出了葡萄球菌和马拉塞西亚
科学领域:
- 微生物学
- 基因组学
- 生物信息学
背景情况:
- 皮肤微生物组研究受到低微生物生物量,宿主细胞污染和RNA不稳定性的挑战.
- 现有的转录组学方法需要改善临床适用性和精确的微生物基因表达特征.
研究的目的:
- 开发一个强大的和临床上适用的皮肤转录学工作流程.
- 在不同的身体部位比较皮肤微生物组的转录和基因组概况.
- 在现场确定活跃的微生物物种,它们的功能和物种间的相互作用.
主要方法:
- 开发一种新的皮肤转录学协议.
- 在27名健康成年人的皮肤部位上配对应用metatranscriptomics和metagenomics.
- 微生物RNA和DNA的物种和基因分析.
主要成果:
- 新的工作流显示出高技术可重复性,均的基因体覆盖,以及丰富的微生物mRNA.
- 在转录组和基因组丰度之间观察到显著的差异,其中Staphylococcus和Malassezia在转录组中占比过高.
- 识别微生物适应特征,多种抗微生物基因表达 (包括细菌素) 和介导微生物相互作用的基因.
结论:
- 开发的皮肤转录组工作流程是稳健的,并且在临床上可操作.
- 转录基因组学提供了对活跃的微生物群落和基因组数据不同功能的洞察.
- 这种方法揭示了微生物在皮肤上的适应性,抗微生物防御和复杂的微生物相互作用.
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