来自Campylobacter jejuni脂质体的 lysophospholipid 的原子模型
Kahlan E Newman1, Astrid F Brandner2, Jonathan W Essex1
1School of Chemistry, University of Southampton, Southampton, UK.
Biophysical journal
|August 29, 2025
概括
在Campylobacter jejuni膜中,尤其是应激状态下,酸是至关重要的. 我们的模型显示这些脂质改变了膜结构,
科学领域:
- 微生物学
- 生物物理
- 计算生物学
背景情况:
- 溶脂是有机体中的重要脂质,包括细菌脂质组的很小部分,但在压力下Campylobacter jejuni中含有很大数量.
- 细菌膜的准确建模需要结合溶解脂,因为它们在C. jejuni中更为普遍.
研究的目的:
- 开发和验证四种C. jejuni脂的原子模型.
- 研究模仿C. jejuni的混合脂-脂双层的自我组装和特性.
- 评估 lysophospholipids 对膜结构,蛋白质嵌入和电穿孔易感性的影响.
主要方法:
- 对C. jejuni脂的原子模型的开发
- 分子动力学模拟以评估相位行为 (状,六角形,状) 和双层性质.
- 模拟混合双层与脂和光脂,包括嵌入膜蛋白.
- 对双层稀释,每脂质面积,脂质扩散和电穿孔的分析.
主要成果:
- 光脂模型成功地重现了预期的脂质阶段.
- 与纯脂双层相比,混合双层自组装,显示微妙的稀释和减少的每脂层面积.
- 利索脂没有显著改变脂质扩散,但促进了孔隙形成,增加了膜对电孔的敏感性.
- 嵌入式蛋白质模拟证明了该模型能够表示复杂的膜系统.
结论:
- 开发的光脂模型准确地代表了C. jejuni的膜成分.
- 溶解脂对膜的生物物理性质有显著影响,特别是增加了对电穿孔的敏感性.
- 这些发现对于精确的C. jejuni膜计算建模和理解它们独特的脂质复杂性至关重要.
更多相关视频
12:57Isolation and Chemical Characterization of Lipid A from Gram-negative Bacteria
Published on: September 16, 2013
32.0K
11:59Isolation of Lipoprotein Particles from Chicken Egg Yolk for the Study of Bacterial Pathogen Fatty Acid Incorporation into Membrane Phospholipids
Published on: May 15, 2019
9.8K
相关概念视频
Formation of Lipopolysaccharides
97
Lipopolysaccharides (LPS) are crucial components of the outer membrane of Gram-negative bacteria, serving both structural and functional roles. It contributes to membrane stability and protects bacteria from host immune responses. LPS is composed of three major regions—lipid A, a core oligosaccharide, and an O antigen. The biosynthesis and assembly of LPS involve a highly coordinated set of enzymatic reactions and transport mechanisms. Additionally, LPS is recognized as an endotoxin,...
97
Structure of Lipids
90.8K
Lipids include a diverse group of compounds that are largely nonpolar in nature. This is because they are hydrocarbons that include mostly nonpolar carbon-carbon or carbon-hydrogen bonds. Non-polar molecules are hydrophobic (“water fearing”), or insoluble in water. Lipids perform many different functions in a cell. Cells store energy for long-term use in the form of fats. Lipids also provide insulation from the environment for plants and animals. For example, they help keep aquatic...
90.8K
Assembly of the Lipid Bilayer in the ER
3.3K
Biological membranes are more than just a barrier separating cell cytoplasm from the outside environment. They are highly dynamic and help maintain the integrity and physiological stability of the cells as well as membrane-bound organelles. Membranes also play vital roles in cell-to-cell and intracellular communication.
A large chunk of any biological membrane is composed of phospholipids. These lipids have a heterogeneous distribution across different subcellular organelles and even between...
A large chunk of any biological membrane is composed of phospholipids. These lipids have a heterogeneous distribution across different subcellular organelles and even between...
3.3K
Asymmetric Lipid Bilayer
7.7K
Biological membranes show uneven distribution of different types of lipids in the inner and outer layers, resulting in transverse asymmetric membranes. The treatment of the erythrocyte membrane with the enzyme phospholipase confirmed the asymmetric nature of the lipid bilayer. The enzyme hydrolyzes lipids into fatty acids and hydrophilic groups. The phospholipase acts only on the outer layer of the membrane, while the inner layer remains intact. The phospholipase treatment resulted in 80%...
7.7K
Membrane Lipids
25.8K
Lipids are an essential component of all biological membranes. The average lipid content in mammalian membranes is 50%, though it can be as low as 20% in the inner mitochondrial membrane or as high as 80% in the myelin sheath present around the nerve cells.
Phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and sphingomyelin are the most common phospholipids present in mammalian membranes. At physiological pH, phosphatidylserine is negatively charged, while the other three...
Phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and sphingomyelin are the most common phospholipids present in mammalian membranes. At physiological pH, phosphatidylserine is negatively charged, while the other three...
25.8K
Biosynthesis of Lipids
91
Microbial membranes exhibit remarkable diversity in lipid composition, reflecting evolutionary adaptations to various environmental conditions. The three domains of life—Bacteria, Archaea, and Eukarya—synthesize membrane lipids through distinct biosynthetic pathways, leading to fundamental structural differences that impact membrane stability, function, and adaptability.Fatty Acid-Based Lipids in Bacteria and EukaryaBacteria and eukaryotes share a common fatty acid biosynthesis...
91
