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测色点诊断监测引起尿路感染的菌

Maria M Morim¹, Patrick Rupper², Stefanie Altenried³

¹Empa, Swiss Federal Laboratories for Materials Science and Technology, Laboratory for Biomimetic Membranes and Textiles, Lerchenfeldstrasse 5, CH-9014, St. Gallen, Switzerland. giorgia.giovannini@marionegri.it.

Journal of materials chemistry. B

|August 29, 2025

概括

一个新的测色传感器通过准它的L-乳酸代谢物来检测. 这种快速诊断工具为尿路感染提供了比传统的尿培养方法更快,更准确的替代方案.

科学领域:

生物医学工程
纳米技术
临床诊断

背景情况:

尿路感染很常见,诊断依赖于缓慢培养方法.
护理点 (POC) 设备为快速诊断和改善治疗提供了潜力.
目前的尿道感染诊断方法耗时且昂贵.

研究的目的:

开发一种快速,精确和准确的色度传感器,用于检测尿液中的菌.
通过准其代谢物L-乳酸盐来间接检测E. faecalis.
创建一个用于定性和定量性尿路感染诊断的传感器.

主要方法:

使用载有MnO2 (MnSNP) 的二氧化纳米粒子 (SNP) 开发了一种传感探测器,并使用乳酸氧化酶 (Lac@MnSNP) 进行功能化.
在每个合成步骤中描述探针形态和组成.
在各种尿样中评估传感器对L-乳酸盐的反应和检测E. faecalis.

主要成果:

Lac@MnSNP 探测器显示信号线性降低至 50 μM L- 乳酸盐,检测极限为 31 μM.
在5小时内成功检测到人工和复杂尿样中的E. faecalis,其度低至10^3 CFU mL^1.
证实纳米粒子形态不受MnO2结合和酶功能化的影响.

结论:

开发的 Lac@MnSNP 探针能够快速,准确和乳酸特异性检测E. faecalis.
这种传感器显示出治疗点尿路感染诊断的巨大潜力.
探测器提供了一个有希望的替代传统,耗时的基于文化的方法.

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