Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Circadian Rhythms and Gene Regulation02:19

Circadian Rhythms and Gene Regulation

The biological clock is involved in many aspects of regulating complex physiology in all animals. It was in 1935 when German zoologists, Hans Kalmus and Erwin Bünning, discovered the existence of circadian rhythm in Drosophila melanogaster. However, the internal molecular mechanisms behind the circadian clock remained a mystery until 1984, when Jeffrey C. Hall, Michael Rosbash, and Michael W. Young discovered the expression of the Per gene oscillating over a 24-hour cycle. In subsequent years,...
Replicative Cell Senescence02:15

Replicative Cell Senescence

Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds the telomeric...
Replicative Cell Senescence02:15

Replicative Cell Senescence

Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds the telomeric...

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Early α-synuclein-mediated mitochondrial dysfunction in a human cell model of Parkinson's disease dementia.

Communications biology·2026
Same author

Improving Face Age Prediction by Using Multiple-Angle Photos.

Computational and structural biotechnology journal·2026
Same author

Physical Fitness Is Negatively Associated With DNA Methylation-Based Risk of Aging-Related Diseases.

Aging cell·2026
Same author

Levels of aggregation of proteins related to mental illness, assayed by insolubility, vary across the brains of individuals.

Scientific reports·2026
Same author

Loss of SETDB1-mediated H3K9me3 in human neural progenitor cells leads to transcriptional activation of L1 retrotransposons.

Nucleic acids research·2026
Same author

The evolution of gene regulation in mammalian cerebellum development.

Science (New York, N.Y.)·2026

相关实验视频

Updated: May 12, 2026

Gentle Isolation of Nuclei from the Brain Tissue of Adult African Turquoise Killifish, a Naturally Short-Lived Model for Aging Research
09:28

Gentle Isolation of Nuclei from the Brain Tissue of Adult African Turquoise Killifish, a Naturally Short-Lived Model for Aging Research

Published on: August 9, 2022

2.2K

基于单核转录学的人类大脑细胞类型特定的衰老时钟

Chandramouli Muralidharan1,2,3, Enikő Zakar-Polyák4,5,6, Anita Adami1

  • 1Laboratory of Molecular Neurogenetics, Department of Experimental Medical Science, Wallenberg Neuroscience Center and Lund Stem Cell Center, Lund University, Lund, 221 84, Sweden.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)
|August 29, 2025
PubMed
概括

科学家们使用人类大脑数据开发了特定细胞类型的转录性衰老时钟. 这些时钟准确地测量了不同脑细胞的生物衰老, 揭示了神经退行性疾病的改变.

关键词:
陈旧的时钟生物钟人类大脑衰老单核测序转录组时钟

更多相关视频

Fluorescence-Activated Nuclei Negative Sorting of Neurons Combined with Single Nuclei RNA Sequencing to Study the Hippocampal Neurogenic Niche
08:16

Fluorescence-Activated Nuclei Negative Sorting of Neurons Combined with Single Nuclei RNA Sequencing to Study the Hippocampal Neurogenic Niche

Published on: October 20, 2022

3.1K
Author Spotlight: Advancing Biomedical Research Through Single Cell Analysis
07:59

Author Spotlight: Advancing Biomedical Research Through Single Cell Analysis

Published on: December 22, 2023

2.8K

相关实验视频

Last Updated: May 12, 2026

Gentle Isolation of Nuclei from the Brain Tissue of Adult African Turquoise Killifish, a Naturally Short-Lived Model for Aging Research
09:28

Gentle Isolation of Nuclei from the Brain Tissue of Adult African Turquoise Killifish, a Naturally Short-Lived Model for Aging Research

Published on: August 9, 2022

2.2K
Fluorescence-Activated Nuclei Negative Sorting of Neurons Combined with Single Nuclei RNA Sequencing to Study the Hippocampal Neurogenic Niche
08:16

Fluorescence-Activated Nuclei Negative Sorting of Neurons Combined with Single Nuclei RNA Sequencing to Study the Hippocampal Neurogenic Niche

Published on: October 20, 2022

3.1K
Author Spotlight: Advancing Biomedical Research Through Single Cell Analysis
07:59

Author Spotlight: Advancing Biomedical Research Through Single Cell Analysis

Published on: December 22, 2023

2.8K

科学领域:

  • 神经科学
  • 基因组学
  • 老龄化研究

背景情况:

  • 衰老是神经退行性疾病的主要危险因素.
  • 了解特定细胞类型的大脑衰老至关重要,

研究的目的:

  • 开发和验证特定于人类细胞类型的转录组衰老时钟.
  • 研究人类大脑中特定细胞类型的衰老模式.
  • 在神经退行性疾病中探索改变的衰老轨迹.

主要方法:

  • 使用了死后人类前额叶皮层的单核RNA测序数据 (31名18-94岁的捐赠者).
  • 在主要脑细胞类型上训练的转录基因衰老时钟.
  • 在独立数据集上验证了时钟性能.

主要成果:

  • 在不同细胞类型中鉴定出与衰老相关的显著转录基因变化,包括微质中的炎症基因上调.
  • 时间表准确地预测了年龄, 并捕捉了生物相关途径.
  • 在独立的数据集中证明了时钟的稳定性.
  • 在阿尔茨海默病和精神分裂症患者中观察到特定细胞类型的衰老加速.

结论:

  • 细胞类型特定的转录原子钟是测量人类大脑生物衰老的可行工具.
  • 这些时钟可以识别神经退行性疾病的衰老轨迹.
  • 这些发现突出了神经疾病中选择性脆弱性的潜在机制.