细胞内性 Pseudomonas oryzihabitans CB24 通过单酸糖驱动的脂质重编程促进光合作用
Nikky Deepa1,2, Shivam Chauhan1,2, Prabodh K Trivedi2,3
1Division of Crop Production and Protection, CSIR-Central Institute of Medicinal and Aromatic Plants, Lucknow, India.
Plant, cell & environment
|August 29, 2025
概括
内生细菌Pseudomonas oryzihabitans通过促进脂质代谢和精油的产生来增强Pelargonium graveolens的生长. 这种微生物协会改善了植物生理和植物化学,提高了产量.
科学领域:
- 植物与微生物的相互作用
- 植物内生微生物学
- 植物生物化学
背景情况:
- 这种植物因其精油而受到重视.
- 内生协会会影响植物生理和植物化学.
- 细胞内介导调节的机制在很大程度上尚未被探索.
研究的目的:
- 调查 Pseudomonas oryzihabitans CB24 疫苗对P. graveolens 的影响.
- 阐明植物新陈代谢中内生菌诱导的变化的机制.
- 了解内植物如何调节植物生长和产量.
主要方法:
- 用P. oryzihabitans CB24对P. graveolens进行注射
- 对植物脂质动态和基因表达的分析.
- 测量酶活性和代谢物积累.
主要成果:
- 通过增强前体合成和对关键代谢基因 (plc,MGD1,SQD2) 的上调,P. oryzihabitans CB24增加了质糖脂 (MGDG,SQDG).
- 观察到参与光采集复合体 (LHCA2,LHCA1,PsaA,PetE,PetF,PsbO) 的基因升级,并增加了RuBisCO活性.
- 增强DXS基因的表达导致单类产量增加 (geraniol, linalool),使新陈代谢转向二次生物合成.
结论:
- 内生P. oryzihabitans CB24显著改变了P. graveolens的脂质代谢和二次代谢物的产生.
- 植物内增强了植物生理过程,包括酸盐吸收,碳同化和抗氧化能力.
- 这种微生物的结合使宿主植物的生长,发育和精油产量大大提高.
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