在人类头发中检测梅尔多,一种兴奋剂
Pascal Kintz1,2, Jean-Claude Alvarez3, Laurie Gheddar2
1X-Pertise Consulting, Mittelhausbergen, France.
Drug testing and analysis
|August 29, 2025
概括
现在可以在头发中检测出一种由世界反兴奋剂机构禁止的增强性药物梅尔多尼. 这项研究开发了一种识别头发样本中的梅尔多的方法,有助于反兴奋剂调查.
科学领域:
- 药理学
- 分析化学
- 运动科学
背景情况:
- 梅尔用于治疗心血管疾病,但运动员滥用它来提高表现.
- 自2016年以来,世界反兴奋剂机构已禁止使用梅尔多,因为它具有代谢调节特性.
- 检测头发中的梅尔多对于反兴奋剂工作至关重要,
研究的目的:
- 开发和验证一种检测人类头发中的梅尔多的方法.
- 建立一个可靠的分析技术来确定运动员的梅尔多暴露.
- 提供证据证明梅尔多被添加到头发中以进行兴奋剂控制.
主要方法:
- 使用甲醇化和超声波辅助洗从头发样本中提取 meldonium.
- 使用液体染色学与双重质谱法 (LC-MS/MS) 分离和检测梅尔多.
- 对分析方法的线性 (0.5100 pg/mg,R2 = 0.9943) 和检测极限 (0.1 pg/mg) 的验证.
主要成果:
- 开发的方法在头发样本中成功提取并检测出梅尔多.
- 在三个消费者的近端头发段 (01厘米) 中发现了梅尔.
- 检测到的度为0. 7,6. 1和17 pg/ mg,证实了头发的结合.
结论:
- 这项研究证明了梅尔多成功地被纳入人类头发中.
- 经过验证的LC-MS/MS方法提供了检测运动员滥用梅尔多尼的工具.
- 毛发分析提供了一种有价值的方法来记录长期的梅尔多暴露在兴奋剂控制中.
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