PFKFB3-介导的糖分代谢重编程 调节干眼疾病的炎症反应
Kaiye Zhang1, Yu Zhang1,2, Xiaojie Wan1
1Eye Center, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Zhejiang Provincial Key Laboratory of Ophthalmology, Zhejiang Provincial Clinical Research Center for Eye Diseases, Zhejiang Provincial Engineering Institute on Eye Diseases, Hangzhou, China.
Investigative ophthalmology & visual science
|August 29, 2025
概括
干眼病 (DED) 通过PFKFB3驱动的糖解引起炎症,激活NF-κB通路. 通过破坏这种代谢-炎症循环,针对PFKFB3提供了潜在的新疗法.
科学领域:
- 眼睛表面研究
- 代谢途径
- 炎症生物学
背景情况:
- 干眼病 (DED) 的特征是眼睛表面的炎症.
- 葡萄糖溶解和炎症途径与DED的发病有关.
- 特定的糖分调节剂在DED中的作用尚不清楚.
研究的目的:
- 调查DED中的糖分和炎症变化.
- 探索6-果-2-激酶/果糖-2,6-二酸酶3 (PFKFB3) -依赖糖解对核因子kappa B (NF-κB) 途径的调节作用.
主要方法:
- 人类角膜上皮细胞 (HCECs) 在试验室中受到高度应激,以模拟DED.
- 通过RT-qPCR和乳酸测定,评估了糖解基因表达和乳酸分泌.
- 转录组测序确定了关键的调节基因;PFKFB3操纵 (过度表达/抑制) 验证了其在糖解和NF-κB激活中的作用.
主要成果:
- 超的压力增加了HCEC中的糖解酶,乳酸生产和炎症细胞因子.
- 转录组学发现PFKFB3和NF-κB通路基因上调.
- 抑制PFKFB3可以逆转糖解变化,抑制NF- kB酸化,并减少TNF- α的分泌.
结论:
- 超的压力通过PFKFB3介导的糖分分解重编程激活NF-κB通路,从而产生代谢-炎症循环.
- 向PFKFB3可能会破坏这种循环,并为DED提供一种新的治疗策略.
相关概念视频
Inflammatory Response
7.0K
An inflammatory response is a localized, nonspecific immune reaction that occurs when a tissue is injured. It is characterized by redness, swelling, heat, and pain, which are commonly called the cardinal signs and symptoms of inflammation. Inflammation can sometimes result in a loss of function.
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
Inflammation can be triggered by various stimuli, such as impact, abrasion, chemical irritation, infections, and extreme hot or cold temperatures. These can damage cells and connective tissue fibers,...
7.0K
Inflammatory Response II: Inflammatory Exudate and Tissue Repair
5.6K
The immune system's inflammatory response destroys the invading pathogen, permitting the tissue to heal. The changes during the cellular and vascular stages allow exudate formation at the site of inflammation. The inflammatory exudate released from the wound has high protein content and a specific gravity above 1.020.
The typical wound exudate is odorless, transparent, straw-colored, thin, and watery. Exudate, however, can differ depending on the state of wound healing. Likewise, the...
The typical wound exudate is odorless, transparent, straw-colored, thin, and watery. Exudate, however, can differ depending on the state of wound healing. Likewise, the...
5.6K
Inflammatory Response I: Vascular and Cellular
12.4K
The inflammatory response is the body's defense against infection, injury, or irritation from bacteria, trauma, toxins, or heat. Inflammation helps locate and destroy pathogens and remove damaged tissue elements to heal the body. During this initial phase, fluid, blood products, and nutrients migrate to the injured area, resulting in redness, heat, swelling, ache, and loss of function. Moreover, signs of systemic inflammation include fever, increased WBC count, malaise, anorexia, nausea,...
12.4K


