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相关概念视频

RNA-seq03:21

RNA-seq

10.4K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
10.4K
Ribosome Profiling02:24

Ribosome Profiling

3.6K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
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Small-Molecule Degradation of the MicroRNA-21 Precursor Rescues Pathogenic Pathways in Cellular Models of Fibrosis.

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Comparison of temporary skeletal anchorage device-assisted maxillary molar distalization between clear aligners and fixed appliances: A retrospective cone-beam computed tomography study.

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Mechanistically Defined Epoxide- and Aziridine-2-carboxamide Electrophiles Enable Stereoselective Covalent Ribonucleic Acid Modulation.

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Lipid Metabolic Labeling to Study Site- and Lipid-Specific Long-Chain <i>S</i>-Acylation Dynamics.

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Inositol Thiophosphates as Inhibitors of Mammalian, Plant, and Fungal Phytases.

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Synthesis and Characterization of the Spectroscopic and Imaging Utilities of Two Indole-Based Cyan Fluorescent Nucleoside Analogues.

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Indole Ring Expansion and Rearrangement-Enabled Quinoline Scaffold Formation in the Biosynthesis of the Antitumor Monoterpene Indole Alkaloid Camptothecin.

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相关实验视频

Updated: Sep 9, 2025

NMR-Based Fragment Screening in a Minimum Sample but Maximum Automation Mode
09:19

NMR-Based Fragment Screening in a Minimum Sample but Maximum Automation Mode

Published on: June 4, 2021

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精简的基于碎片的发现平台用于准结构RNA

Yilin Jia1,2, Amirhossein Taghavi1, Patrick R A Zanon1

  • 1Department of Chemistry, The Herbert Wertheim UF Scripps Institute for Biomedical Innovation & Technology, 130 Scripps Way, Jupiter, Florida 33458, United States.

ACS chemical biology
|August 29, 2025
PubMed
概括
此摘要是机器生成的。

这项研究引入了针对RNA的基于片段的新药发现平台. 它使用新的化学标签可视化和优化小分子,

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RNA Secondary Structure Prediction Using High-throughput SHAPE
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RNA Secondary Structure Prediction Using High-throughput SHAPE

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Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

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相关实验视频

Last Updated: Sep 9, 2025

NMR-Based Fragment Screening in a Minimum Sample but Maximum Automation Mode
09:19

NMR-Based Fragment Screening in a Minimum Sample but Maximum Automation Mode

Published on: June 4, 2021

3.4K
RNA Secondary Structure Prediction Using High-throughput SHAPE
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RNA Secondary Structure Prediction Using High-throughput SHAPE

Published on: May 31, 2013

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Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions
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Sample Preparation for Mass Spectrometry-based Identification of RNA-binding Regions

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科学领域:

  • 生物化学
  • 化学生物学
  • 药物发现

背景情况:

  • 基于碎片的药物发现 (FBDD) 传统上使用光谱方法.
  • 识别与生物分子的低亲和性结合剂对于FBDD至关重要.
  • 用小分子准RNA面临着独特的挑战.

研究的目的:

  • 开发针对RNA的小分子简化基于片段的选平台.
  • 展示这个新平台的概念验证.
  • 识别与疾病相关的特定RNA结构结合的片段.

主要方法:

  • 使用了低分子量碎片与迪亚齐林和标签.
  • 采用光解,然后使用光体进行点击化学,以可视化.
  • 对与1型肌性衰变相关的r(CUG) 重复扩张RNA进行选.

主要成果:

  • 在目标RNA中与1x1核酸U/U内部循环结合的已识别的片段.
  • 设计的同位体化合物与相邻的内部循环相互作用.
  • 开发了一种具有增强亲和力的二元化合物, 转化为共价结合剂,

结论:

  • 建立了一个针对结构化RNA的多功能平台.
  • 证明了开发针对RNA标的治疗方法的潜力.
  • 该平台对各种疾病相关的RNA标具有广泛的应用.