通过系统优化的触流超和纳米圈微生物组颗粒回收病毒组序列的废水RNA
Emily Segelhurst1, Jonathan E Bard2,3,4, Sydney Gallo1
1Department of Civil, Structural and Environmental Engineering, University at Buffalo, Buffalo, New York, USA.
Applied and environmental microbiology
|August 29, 2025
概括
污水处理方法如触流超过 (TFF) 和纳米陷粒子产生类似的SARS-CoV-2测序结果,但丰富了不同的RNA病毒组. 选择方法对于精确的废水基因组监测至关重要.
科学领域:
- 环境病毒学
- 基因组流行病学
- 基于废水的流行病学
背景情况:
- 市政废水含有多种RNA病毒,对人类,动物和植物健康构成风险.
- 废水的基因组测序对于追踪病毒病原体和了解社区健康至关重要.
- 废水处理方法对RNA病毒组测序结果产生重大影响.
研究的目的:
- 系统地评估触流超 (TFF) 和纳米圈微生物组A颗粒用于废水中的RNA病毒检测和表征.
- 通过amplicon测序来比较TFF和Nanotrap方法在检测严重急性呼吸系统冠状病毒2 (SARS-CoV-2) 的有效性.
- 评估这些方法对RNA病毒的全转录组枪测序的影响.
主要方法:
- 使用废水样本对接流超 (TFF) 和纳米圈微生物组A颗粒的对比.
- 使用安普利康测序检测SARS-CoV-2.
- 使用全转录组射手枪测序对RNA病毒的表征.
主要成果:
- 无论是TFF还是Nanotrap方法都在血统层面发现了类似的SARS-CoV-2变种.
- 优化TFF的度因子和Nanotrap的自动化程序对于高深度基因组覆盖至关重要.
- 这两种方法丰富了不同的RNA病毒组,TFF显示了Reoviridae和Coronaviridae的较高相对丰度.
结论:
- 由于不同的度机制,废水处理方法对RNA病毒组进行了差异丰富.
- 选择和优化方法对于精确的废水病毒基因组监测至关重要.
- 这些发现对废水基因组监测的更广泛的环境应用有影响.
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