在结核病治疗过程中获得的连续采样唾液样本中的污染率
N Niemand1, J A Rooney2, S Malatesta3
1South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa.
Microbiology spectrum
|August 29, 2025
概括
监督的唾液采集和快速的实验室处理显著降低了结核菌种的污染率. 没有监督的样本和较长的处理时间增加了污染,影响了诊断准确度.
科学领域:
- 微生物学
- 传染性疾病
- 公共卫生
背景情况:
- 唾液培养对于结核病的诊断和监测至关重要.
- 液体介质培养 (MGIT) 的微生物污染是一个重大挑战,导致无法解释的结果.
- 之前的研究表明,冷链维护和监督收集减少了污染,但纵向数据有限.
研究的目的:
- 评估处理时间和无监督采集与液培养污染率之间的关系.
- 在12周的结核治疗中估计感染率.
- 确定污染的临床和行为预测因素.
主要方法:
- 分析了301名结核病患者的3,155个唾液样本,经过12周的治疗.
- 根据从采集到加工和采集监督的时间评估污染率.
- 污染与涂抹等级和其他临床/行为因素的相关性.
主要成果:
- 污染率从治疗开始时的12. 3%增加到第8周的30%左右.
- 没有监督的样本采集与更高的污染率相关 (P=0. 048在开始时,P=0. 028超过12周).
- 较低的涂抹等级与更高的污染风险相关.
结论:
- 监督的唾液采集对于尽量减少结核病培养物的污染至关重要.
- 及时的实验室处理和严格的冷链是必不可少的.
- 这些发现对于改善结核病诊断和治疗监测至关重要,尤其是在资源有限的环境中.
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