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Updated: Sep 9, 2025

Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface

Isolation of Leukocytes from the Murine Tissues at the Maternal-Fetal Interface

Published on: May 21, 2015

作为怀孕期间负调节表型的循环免疫细胞

Hong Wang^1,2, Wenting Lu¹, Mengru Zhu³

¹Department of Molecular Biology, College of Basic Medical Sciences, Jilin University, Changchun, Jilin, P.R. China.

Cellular and molecular life sciences : CMLS

|August 29, 2025

概括

在人类怀孕期间,中性粒细胞上的可溶性托尔类受体9 (sTLR9) 降低,影响免疫耐受性. 这种免疫细胞表型转变对于在妊娠期间保持母胎耐受性至关重要.

科学领域:

免疫学
生殖生物学
细胞生物学

背景情况:

收费类受体9 (TLR9) 是细胞表面 (sTLR9) 上也存在的细胞内受体.
sTLR9具有负面的免疫调节作用,促进免疫耐受性.
怀孕涉及炎症以及对胎儿的免疫耐受性,使得怀孕期间免疫细胞的sTLR9变化成为一个关键的研究领域.

研究的目的:

在怀孕期间研究免疫细胞sTLR9表达的变化.
了解 sTLR9 在母胎免疫耐受性中的作用.

主要方法:

孕妇周围血液白细胞 (PWBC) 的分析.
在孕期和非孕期小鼠中对sTLR9水平进行比较研究.
给怀孕的小鼠服用TLR9激动剂 (CpG ODN),以观察免疫反应和后代的结果.

主要成果:

在孕妇中,中性粒细胞百分比的增加与中性粒细胞的sTLR9降低相关 (sTLR9+ Neu%).
与未怀孕的小鼠相比,怀孕小鼠的sTLR9和sTLR9+水平较高,且在三个月内水平有所不同.
在小鼠中,TLR9激动剂诱导了前炎症状态,sTLR9+Neu降低,新生儿体重降低.

结论:

中性粒细胞的sTLR9表达是一种与怀孕期间免疫耐受性相关的动态免疫细胞表型.
sTLR9的变化有助于维持母胎免疫耐受性的机制.
对sTLR9的进一步研究可能为与怀孕相关的免疫调节提供新的见解.

关键词:

免疫耐受性炎症调节周围白细胞 (PWBC)孕妇的免疫反应表皮类似受体9 (sTLR9) 表达的免疫细胞

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