JsSAMDC促进聚胺合成和开花基因以协同调节雌性花分化
Jinyan Chen1,2, Wenwen Li1,2, Chunxiang Li1,2
1College of Agriculture, Guizhou Engineering Research Center for Fruit Crops, Guizhou University, Guiyang, China.
Plant cell reports
|August 29, 2025
概括
在Juglans sigillata中的JsSAMDC1基因通过增加聚胺合成和调节开花基因来促进雌性花的发育. 这项研究解决了这种对产量至关重要的双胞胎植物中雌雄比例较低的问题.
科学领域:
- 植物生殖生物学
- 分子遗传学
- 生物化学
背景情况:
- 核桃是一种双胞胎物种,其雌雄比例较低,限制了作物产量.
- S-adenosylmethionine脱碳酶 (SAMDC) 是聚胺合成中的关键酶,但其在J. sigillata花分化中的作用尚不清楚.
研究的目的:
- 研究JsSAMDC基因在Juglans sigillata中雌性花的分化中的作用.
- 阐明J. sigillata中多氨酸,开花基因和雌性花发育之间的关系.
主要方法:
- 在J. sigillata基因组中识别和表征JsSAMDC基因.
- 对转录组和代谢组数据进行分析,以评估基因协同表达和代谢物水平.
- 对JsSAMDC1的时间空间表达分析.
- 对精子 (Spm) 的外源应用和暂时静止JsSAMDC1以研究基因功能.
主要成果:
- 鉴定了七个JsSAMDC基因;JsSAMDC1与开花基因CO和SOC1显著共同表达.
- 在雌性花中检测到更高的SPM水平,外源性SPM应用增强了开花基因表达.
- 在细胞核,细胞质和血中局部化的 JsSAMDC1 主要表达在雌性花分化过程中.
- 抑制JsSAMDC1降低了聚胺生物合成和与开花相关的基因表达,降低了丁素和精子素,并促进了精子积累.
结论:
- JsSAMDC1和多胺生物合成基因在雌性花中协同增加SPM水平.
- JsSAMDC1,以及开花基因CO和SOC1,促进J. sigillata的雌性花分化.
- 这项研究为了解多胺在J. sigillata雌性花发育中的作用提供了理论基础.
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