通过在线固相提取高性能液体染色体-并联质谱分析血清氨酸
D V Bychenkov1, V A Ioutsi2, I V Kirilina2
1National Medical Research Center for Endocrinology, Ministry of Health of the Russian Federation, Moscow, Russia. bychenkov.denis@endocrincentr.ru.
Bulletin of experimental biology and medicine
|August 29, 2025
概括
我们开发了一种使用HPLC-MS/MS测量血清中的新方法. 这种技术可以准确量化血清水平,有助于诊断血清相关疾病.
科学领域:
- 临床化学
- 分析化学
- 生物化学
背景情况:
- 血清素在各种生理过程中起着至关重要的作用.
- 精确量化血清中的血清激素对于诊断和管理相关疾病至关重要.
- 现有的方法可能面临着矩阵效应和样品制备效率的挑战.
研究的目的:
- 开发和验证一种可靠的方法来定量测定人体血清中的血清.
- 在临床环境中建立可靠的分析技术来评估血清素水平.
- 支持诊断和监测与血清素代谢改变相关的疾病.
主要方法:
- 高性能液体染色学与双重质谱检测 (HPLC-MS/MS).
- 在线固态提取集成到染色系统中,用于自动化样品制备.
- 验证方法的准确性,精度,线性和灵敏度.
主要成果:
- 开发的方法可以在广泛的线性范围 (0.5-2000 ng/ml) 上精确量化血清中的血清素.
- 在线固相提取有效地减少了矩阵组件干扰,提高了测试可靠性.
- 该方法显示了对胺的高灵敏度和特异性.
结论:
- 拟议的HPLC-MS/MS方法提供了一种可靠和有效的血清中血清度测量方法.
- 这种技术有助于定量评估血清水平,这对于临床诊断至关重要.
- 该方法的自动化和减少的矩阵效应使其适用于常规的临床实验室使用和与血清素相关的病理学的研究.
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