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Updated: Sep 9, 2025

Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
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Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry

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网格合干扰仪的使用以研究内酸盐与蛋白质的相互作用

Kristina Sturm1, Michael Hothorn2

  • 1Structural Plant Biology Laboratory, Department of Plant Sciences, University of Geneva, Geneva, Switzerland.

Methods in molecular biology (Clifton, N.J.)
|August 29, 2025
PubMed
概括

网格合干扰计 (GCI) 测量蛋白质 - 连接体结合动力学. 本研究使用生物化异酸盐 (PP-InsP) 的 GCI 来分析PP-InsP蛋白相互作用.

关键词:
结合动力学克雷奥普蒂克斯的浪潮网格合干涉测量无酸酸盐蛋白质与配体的相互作用

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Phosphoinositides and PIPs01:42

Phosphoinositides and PIPs

Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
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