Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.3K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.3K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Tissue-specific targeting of DNA nanodevices in a multicellular living organism.

eLife·2021
查看所有相关文章

相关实验视频

Updated: Sep 9, 2025

Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence TIRF Microscopy
08:55

Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence TIRF Microscopy

Published on: February 17, 2023

3.3K

解码器官离子动态的量化策略

Palapuravan Anees1

  • 1Department of Chemistry, Indian Institute of Science Education and Research (IISER) Tirupati, Tirupati, 517619, India.

Chembiochem : a European journal of chemical biology
|August 29, 2025
PubMed
概括

细胞器官离子动态对于细胞功能和平衡至关重要. 新的成像工具提高了我们对离子调节的理解,帮助疾病研究和治疗目标的识别.

科学领域:

  • 细胞生物学
  • 生物化学
  • 生理学

背景情况:

  • 细胞器官内的离子动力学对于稳态和特定的细胞功能至关重要.
  • 稳定的离子梯度由膜蛋白维持,使有机细胞特异性活动.
  • 离子运输的失调与神经退化和癌症等疾病有关.

研究的目的:

  • 为器官离子动态提供功能成像原理的概述.
  • 突出目前对离子流量进行量化评估的挑战.
  • 讨论器官特异性离子调节的未来方向.

主要方法:

  • 使用先进的成像技术,包括光探针 (例如基于绿色光蛋白),小分子和DNA纳米设备.
  • 使用高空间和时间分辨率的离子运动分析方法.
  • 进行离子传输机制的定性和定量分析.

主要成果:

  • 最近的技术进步显著改善了离子动力学的研究.
  • 新的工具为分析离子流提供了增强的空间和时间分辨率.
  • 这些方法提供了对离子运输的生理相关性的见解.

结论:

关键词:
电池紧光探测器器官的功能成像离子通道和传送器器官离子动力学

更多相关视频

Quantitative Approaches for Studying Cellular Structures and Organelle Morphology in Caenorhabditis elegans
08:47

Quantitative Approaches for Studying Cellular Structures and Organelle Morphology in Caenorhabditis elegans

Published on: July 5, 2019

9.8K
Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes
08:15

Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes

Published on: May 22, 2017

8.2K

相关实验视频

Last Updated: Sep 9, 2025

Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence TIRF Microscopy
08:55

Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence TIRF Microscopy

Published on: February 17, 2023

3.3K
Quantitative Approaches for Studying Cellular Structures and Organelle Morphology in Caenorhabditis elegans
08:47

Quantitative Approaches for Studying Cellular Structures and Organelle Morphology in Caenorhabditis elegans

Published on: July 5, 2019

9.8K
Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes
08:15

Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes

Published on: May 22, 2017

8.2K
  • 了解分子水平的离子调节对于细胞生物学和疾病病理学至关重要.
  • 进步的成像技术是解开器官离子动态的关键.
  • 对器官特异性离子调节的进一步研究可以揭示新的治疗点.