相关实验视频

Updated: Sep 9, 2025

Exosomal miRNA Analysis in Non-small Cell Lung Cancer NSCLC Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool

Exosomal miRNA Analysis in Non-small Cell Lung Cancer NSCLC Patients' Plasma Through qPCR: A Feasible Liquid Biopsy Tool

Published on: May 27, 2016

在imatinib耐药慢性骨髓性白血病患者中的外体miRNA表达概况:试点研究

Raphatphorn Navakanitworakul¹, Pirun Saelue², Tipparat Penglong³

¹Department of Biomedical Sciences and Biomedical Engineering, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand.

|August 29, 2025

概括

研究人员在慢性髓性白血病 (CML) 患者的外体内发现了特定的微RNA (miRNA). 这些血外体miRNAs,包括miR- 451a和miR- 16-2- 3p,可作为CML中伊马替尼抗性的生物标志物.

科学领域:

血液学
分子生物学
癌症学

背景情况:

慢性髓性白血病 (CML) 是一种由BCR-ABL驱动的恶性瘤.
伊马替尼 (IM) 是一种前线疗法,但发生耐药性.
外基因衍生微RNA (miRNA) 涉及药物耐药性,并且可以作为生物标志物.

研究的目的:

在CML患者中研究血外体miRNA概况.
确定与伊马替尼 (IM) 耐药性相关的潜在生物标志物.
探索miRNAs在CML耐药性机制中的作用.

主要方法:

从IM敏感和IM耐性CML患者的血中分离出外体.
使用RNA测序分析出外体miRNA含量.
进行了差异表达分析和生物信息路径分析.

主要成果:

在IM耐药患者中,有13个miRNAs被上调和21个miRNAs被下调.
与自和PI3K-Akt信号传递相关的途径显著增强.
在IM耐药外体中,miR- 451a和miR- 16-2- 3p显著上调.

结论:

血外体miRNAs,特别是miR- 451a和miR- 16-2- 3p,显示为CML中伊马替尼抗性的潜在生物标志物.
这些发现有助于了解CML的耐药性机制.
为了评估临床相关性,需要在更大的群体中进行进一步的验证.

更多相关视频

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile

Isolation and Profiling of MicroRNA-containing Exosomes from Human Bile

Published on: June 13, 2016

MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier MSC for Lung Cancer Screening

MicroRNA Based Liquid Biopsy: The Experience of the Plasma miRNA Signature Classifier MSC for Lung Cancer Screening

Published on: October 26, 2017

相关概念视频

MicroRNAs

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...