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相关概念视频

DNA Topoisomerases02:02

DNA Topoisomerases

32.0K
Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types. ...
32.0K
RNA Structure01:19

RNA Structure

5.2K
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
5.2K
Routh-Hurwitz Criterion I01:15

Routh-Hurwitz Criterion I

332
Consider an electrical power grid, where stability is essential to prevent blackouts. The Routh-Hurwitz criterion is a valuable tool for assessing system stability under varying load conditions or faults. By analyzing the closed-loop transfer function, the Routh-Hurwitz criterion helps determine whether the system remains stable.
To apply the Routh-Hurwitz criterion, a Routh table is constructed. The table's rows are labeled with powers of the complex frequency variable s, starting from the...
332
Conserved Binding Sites01:49

Conserved Binding Sites

4.3K
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
4.3K
Protein Folding Quality Check in the RER01:29

Protein Folding Quality Check in the RER

3.8K
ER is the primary site for the maturation and folding of soluble and transmembrane secretory proteins. The calnexin cycle is a specific chaperone system that folds and assesses the confirmation of N-glycosylated proteins before they can exit the ER lumen. The primary players of this quality check pipeline are the lectins, ER-resident chaperones, and a glucosyl transferase enzyme. In case the calnexin system in the lumen fails to salvage a misfolded protein, it is transported to the cytoplasm...
3.8K
Routh-Hurwitz Criterion II01:19

Routh-Hurwitz Criterion II

400
In the application of the Routh-Hurwitz criterion, two specific scenarios can arise that complicate stability analysis.
The first scenario occurs when a singular zero appears in the first column of the Routh table. This situation creates a division by zero issues. To resolve this, a small positive or negative number, denoted as epsilon (∈), is substituted for the zero. The stability analysis proceeds by assuming a sign for ∈. If ∈ is positive, any sign change in the first...
400

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相关实验视频

Updated: Sep 9, 2025

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues
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Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues

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R-循环语法预测不同拓约束下的 R-循环形成

Margherita Maria Ferrari1, Svetlana Poznanović2, Manda Riehl3

  • 1Department of Mathematics, University of Manitoba, Winnipeg, Manitoba, Canada.

PLoS computational biology
|August 29, 2025
PubMed
概括
此摘要是机器生成的。

这项研究引入了一种新的R循环语法,用于预测R循环的形成,R循环是参与基因调节和疾病的关键核酸结构. 语法准确地预测R循环的发生,即使没有明确的拓数据.

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RNA Secondary Structure Prediction Using High-throughput SHAPE
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RNA Secondary Structure Prediction Using High-throughput SHAPE

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De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

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相关实验视频

Last Updated: Sep 9, 2025

Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues
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Optimization of Synthetic Proteins: Identification of Interpositional Dependencies Indicating Structurally and/or Functionally Linked Residues

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RNA Secondary Structure Prediction Using High-throughput SHAPE
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RNA Secondary Structure Prediction Using High-throughput SHAPE

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De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data
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De novo Identification of Actively Translated Open Reading Frames with Ribosome Profiling Data

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科学领域:

  • 分子生物学
  • 遗传学
  • 生物信息学

背景情况:

  • 在转录过程中形成的三链核酸结构.
  • 它们在基因表达调节中起着至关重要的作用.
  • R环形成受DNA序列和拓学的影响,但机制尚不清楚.

研究的目的:

  • 开发一个R循环形成的预测模型.
  • 使用正式语法表示R循环.
  • 研究DNA拓对R循环预测的影响.

主要方法:

  • 开发R循环语法.
  • 训练单分子R循环足迹和测序 (SMRF-seq) 的实验数据的语法.
  • 在不同拓的等离子体上测试预测精度.

主要成果:

  • 它可以准确地预测R循环的形成.
  • 即使没有明确的拓信息,模型也表现良好.
  • 这种R循环语法比以前的预测方法更好.

结论:

  • 一个正式的语法可以有效地建模和预测R循环的形成.
  • 这种方法为研究R循环动态提供了一个新的工具.
  • R循环语法对理解基因调节和疾病发病有影响.