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Updated: Sep 9, 2025

Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence TIRF Microscopy
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Single-Molecule Imaging of Lateral Mobility and Ion Channel Activity in Lipid Bilayers using Total Internal Reflection Fluorescence TIRF Microscopy

Published on: February 17, 2023

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通过全内部反射显微镜探索微载体的蛋白质冠状介导近壁限制运动

Wei Liu1, Zuwei Zhao1, Jinwei Zhong1

  • 1The Key Laboratory of Synthetic and Biological Colloids, Ministry of Education & School of Chemical and Material Engineering, Jiangnan University Wuxi 214122 P. R. China uvyzhu@gmail.com.

Chemical science
|August 29, 2025
PubMed
概括

蛋白质冠状改变了微粒在表面附近的行为. 这项研究揭示了蛋白质层如何影响受限运动和相互作用,显示了由于水分和弹性的扩散和机械性质的转变.

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Visualization of Cortex Organization and Dynamics in Microorganisms, using Total Internal Reflection Fluorescence Microscopy14:14

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相关概念视频

Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
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