在尼日利亚零售肉类中对Enterococcus faecalis的基因组和生物信息洞察
Charles Ayodeji Osunla1,2, Ayorinde Akinbobola1, Arif Elshafea3
1Department of Microbiology, Adekunle Ajasin University, Akungba Akoko, Nigeria.
International journal of microbiology
|August 29, 2025
概括
在尼日利亚零售肉类,特别是肉中发现的抗菌素耐药的Enterococcus faecalis突出显示了严重的公共卫生风险. 全基因组测序揭示了关键的耐药性基因和移动元素,强调需要加强食品安全监测.
科学领域:
- 微生物学
- 基因组学
- 食品安全问题
背景情况:
- 杆菌是一种主要的机会性病原体,抗菌素耐药性 (AMR) 和动物传染性潜力不断增长.
- 了解E. faecalis在食物来源中的基因组环境对于公共健康至关重要.
- 零售肉类是食物传播病原体的潜在储存库.
研究的目的:
- 通过全基因组测序 (WGS) 来描述尼日利亚零售肉类中的Enterococcus faecalis分离物.
- 确定抗微生物耐药性基因 (ARG),毒性因子和移动遗传元素 (MGE).
- 确定E. faecalis分离物的基因组关系.
主要方法:
- 采集和加工50个生肉样本 (肉,牛肉,火).
- 恢复E. faecalis分离物的抗菌敏感性测试.
- 全基因组测序 (WGS) 和ARG,病毒性基因,MGE和基因组学的生物信息分析.
主要成果:
- 发现了10种E. faecalis分离物,主要来自;所有分离物都对克林达米辛,红色素和四环素有耐药性.
- 占主导地位的ARG包括aac6') -aph2"),ant6) -Ia,lsaA),ermB),tetM和tetL.
- 基因组分析确定了两个主要的血统 (ST477,ST16) 具有地理聚类,并揭示了MGE的ARG和毒性因子的局部化.
结论:
- 通过食物传播的E. faecalis分离物具有多种耐药性和毒性因素,构成动物感染风险.
- 存在MGE可以促进潜在的横向基因转移.
- 特别是在低收入和中等收入国家,对食品系统进行基因组监测和抗菌耐药性控制至关重要.
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