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标准化冷FMT是理想的保护剂吗?
Alix Pourrat1, Vinciane Baillieu1, Solene Ansel1
1Pharmacie à Usage Intérieur, Hôpitaux Universitaires Henri Mondor, Créteil, France.
Frontiers in microbiology
|August 29, 2025
概括
干便微生物移植 (FMT) 需要有效的保护剂. 在冷干燥FMT中,Trehalose 5% (T5) 和高抗氧化剂矩阵 (HM) 有望保持细菌活力.
科学领域:
- 微生物学
- 胃肠病学
- 生物技术
背景情况:
- 便微生物移植 (FMT) 是对复发性*Clostridioides difficile*感染的一种经过验证的治疗方法.
- 干提供了一种实用,标准化的FMT配方,但需要有效保存多种细菌.
- 目前没有一种通用介质可以在冷干燥过程中最佳地保护无氧和有氧细菌.
研究的目的:
- 评估干便微生物移植 (FMT) 的新型保护剂.
- 为了比较胰岛素和葡萄糖胺 (IG5) 和高抗氧化剂矩阵与三糖 (HM) 与5%三糖 (T5) 的疗效.
主要方法:
- 在冷干燥后使用殖民地形成单位 (CFU) 计数来评估细菌活力.
- 使用LIVE/DEADTM染色的流细胞测量来确定细菌的活力.
- 对不同防护剂配方的细菌恢复率进行比较分析.
主要成果:
- 对无气体生物 (84±28%) 和*Bifidobacterium* (59±39%) 进行了令人满意的活力.
- 具有高抗氧化剂的三糖 (HM) 显示了最高的活力 (91±7%无气体,121±33%*Bifidobacterium*),但并没有明显超过T5.
- 胰岛素和葡萄糖胺 (IG5) 导致显著的细菌损失 (16±12%的无氧菌,19±9%的*Bifidobacterium*).
结论:
- HM和T5都是冷干燥FMT的有效保护剂,而HM的回收率略高.
- T5提供一致的结果,可以作为独立的保护剂或改进配方的基础.
- 由于显著的细菌损失,IG5不适合使用;对于优化的冷口服FMT囊,需要进一步的研究.
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