通过代谢和过程优化,合成微生物联盟提高了的乙产量和功能质量
Yerui OuYang1,2, Shaoqing Zou1,2, Panpan Liu1,2
1Key Laboratory of Natural Microbial Medicine Research of Jiangxi Province, College of Life Sciences, Jiangxi Science and Technology Normal University, Nanchang, China.
Frontiers in microbiology
|August 29, 2025
概括
一个新的微生物联盟 Lactobacillus plantarum 和 Acetobacter pasteurianus 在中显著增加了乙的产生. 这种高产的发酵增强了的味道,并增加了有益的化合物.
科学领域:
- 食品科学与技术
- 微生物学
- 生物技术
背景情况:
- 乙 (3-基-2-butanone) 是水果中重要的风味化合物.
- 目前在中生产乙的方法可能缺乏效率和产量.
- 优化微生物协会可以增强关键风味化合物的生物合成.
研究的目的:
- 开发一种高产量的酸发酵工艺,使用合成微生物联盟.
- 调查共同培养系统中增强乙生产的机制.
- 评估该工艺对的整体质量和功能成分的影响.
主要方法:
- 选和结合乳酸菌植物 NF2 和乳酸菌 NF171 进行共同培养发酵.
- 优化发酵参数,包括注射剂大小,,温度和糖补充.
- 使用转录组分析来了解乙生物合成的分子机制.
- 在发酵中量化乙,酸,酸和黄.
主要成果:
- 与单一培养相比,共同培养系统的乙度显著提高 (4033.72 mg/L),增加了八倍.
- 优化条件包括3%的注射剂,120rpm的,33°C和20g/L的糖补充剂.
- 转录组分析表明,NADH代谢流量下调,α-乙酸盐合成酶基因上调.
- 这种工艺使富含酸 (57.91g/L),酸和黄.
结论:
- 一个L. plantarum和A. pasteurianus的合成微生物联盟提供了一个可扩展的策略,用于在中产生高产量的乙.
- 这种共同培养方法增强了味道,丰富了功能性化合物,提高了的质量和潜在的健康益处.
- 微生物联盟的合理设计和工艺工程对于改善功能性食品生产是有效的.
更多相关视频
相关概念视频
Microbial Fermentation
292
Fermentation is a crucial anaerobic metabolic process that enables microbes to derive energy from sugar without relying on oxygen or an electron transport chain. This process is fundamental to various biological and industrial applications and is classified based on the metabolic products generated.Role of Pyruvate in FermentationPyruvate and its derivatives serve as key electron acceptors in fermentative pathways. The oxidation of NADH to regenerate NAD+ is essential for the continuation of...
292
Products of the Citric Acid Cycle
99.9K
The cells of most organisms—including plants and animals—obtain usable energy through aerobic respiration, the oxygen-requiring version of cellular respiration. Aerobic respiration consists of four major stages: glycolysis, pyruvate oxidation, the citric acid cycle, and oxidative phosphorylation. The third major stage, the citric acid cycle, is also known as the Krebs cycle or tricarboxylic acid (TCA) cycle.
99.9K
The Citric Acid Cycle
153.4K
The citric acid cycle, also known as the Krebs cycle or TCA cycle, consists of several energy-generating reactions that yield one ATP molecule, three NADH molecules, one FADH2 molecule, and two CO2 molecules.
153.4K
The Citric Acid Cycle: Output
8.4K
The citric acid cycle is termed an amphibolic pathway as it operates both anabolically and catabolically. The cyclic reactions balance the flux of the substrates to provide an optimal concentration of NADH and ATP to the cell.
Regulation of Citric Acid Cycle
The citric acid cycle is regulated in several ways, including feedback inhibition, regulation of enzyme activities, and associated anaplerotic or cataplerotic pathways.
The primary substrate of the TCA cycle—acetyl CoA—is...
Regulation of Citric Acid Cycle
The citric acid cycle is regulated in several ways, including feedback inhibition, regulation of enzyme activities, and associated anaplerotic or cataplerotic pathways.
The primary substrate of the TCA cycle—acetyl CoA—is...
8.4K
The Citric Acid Cycle: Overview
19.3K
In aerobic organisms, the citric acid cycle is the second stage of cellular respiration wherein molecules derived from the breakdown of carbohydrates, proteins, and fats are oxidized into carbon dioxide and energy. This process is also known as the tricarboxylic acid (TCA) cycle as the first product of the cycle, citric acid, contains three carboxyl groups in its structure. Alternatively, this cycle is also referred to as the Krebs cycle, in honor of its discoverer Sir Hans Krebs.
The citric...
The citric...
19.3K
Biosynthesis in Bacteria
106
Biosynthesis in bacteria is a fundamental anabolic process that generates essential macromolecules, including proteins, nucleic acids, lipids, and polysaccharides. These macromolecules are critical for cellular growth, replication, and function. The process is tightly regulated and energetically linked to catabolic pathways to ensure optimal resource utilization.Biosynthetic pathways begin with precursor metabolites such as pyruvate, acetyl-CoA, and glucose-6-phosphate derived from glycolysis,...
106


