桥式亚烯表现出完全可逆的光控制结合到G-四重体DNA/双重体结合
Javier Ramos-Soriano1, Y Jennifer Jiang1, Bowen Deng1,2
1School of Chemistry, University of Bristol, Cantock's Close, Bristol BS8 1TS, United Kingdom.
JACS Au
|August 29, 2025
概括
研究人员开发了一种光控制的分子,可选择性地与特定的DNA结构结合,如HIV-1 LTR-III G-四重复 (G4). 这一突破使得使用可见光对DNA结合进行可逆控制,
科学领域:
- 分子生物学
- 生物化学
- 纳米技术
背景情况:
- 对生物应用和纳米设备来说, 控制外在刺激的DNA构造至关重要.
- 亚衍生物是已知的可光切换分子,但它们在生理条件下的精确DNA向应用具有挑战性.
研究的目的:
- 开发一种能够对特定DNA结构进行可逆光控制的新化学型.
- 调查这种化学型的潜在向G-四重复 (G4) DNA,例如HIV-1 LTR-III序列.
主要方法:
- 一个循环亚博衍生的化学型的合成.
- 在生理条件下使用可见光的光异构化研究.
- 用于确定LTR-III G4的亲和力和选择性进行DNA结合测试.
- 核磁共振 (NMR) 和分子动力学 (MD) 模拟以阐明结合模式.
主要成果:
- 开发的化学型物使用可见光在cis和trans异构体之间进行光切换.
- 与cis异构体相比,trans异构体对LTR-III G4的亲和力是50倍以上的.
- 通过蓝光和绿光照射可逆控制与LTR-III G4的结合和解离.
- 核磁共振和MD模拟显示分别在G4/双重结和双重区域的cis和trans同位素具有不同的结合模式.
结论:
- 一个新的可切换光的分子可以选择性和可逆性地控制G-四重复的DNA结合.
- 这项技术有望开发光激活核酸基纳米设备和针对HIV-1 LTR-III等G4结构的治疗策略.
相关概念视频
Aryldiazonium Salts to Azo Dyes: Diazo Coupling
3.1K
The reaction of weakly electrophilic aryldiazonium (also called arenediazonium) salts with highly activated aromatic compounds leads to the formation of products with an —N=N— link, called an azo linkage. This reaction, presented in Figure 1, is known as diazo coupling and occurs without the loss of the nitrogen atoms of the aryldiazonium salt. Highly activated aromatic compounds such as phenols or arylamines favor the diazo coupling reaction. The coupling generally occurs at the...
3.1K
Photochemical Electrocyclic Reactions: Stereochemistry
1.9K
The absorption of UV–visible light by conjugated systems causes the promotion of an electron from the ground state to the excited state. Consequently, photochemical electrocyclic reactions proceed via the excited-state HOMO rather than the ground-state HOMO. Since the ground- and excited-state HOMOs have different symmetries, the stereochemical outcome of electrocyclic reactions depends on the mode of activation; i.e., thermal or photochemical.
Selection Rules: Photochemical Activation
Selection Rules: Photochemical Activation
1.9K
Single-Strand DNA Binding Proteins
14.9K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
14.9K
Cycloaddition Reactions: MO Requirements for Photochemical Activation
2.2K
Some cycloaddition reactions are activated by heat, while others are initiated by light. For example, a [2 + 2] cycloaddition between two ethylene molecules occurs only in the presence of light. It is photochemically allowed but thermally forbidden.
2.2K


