通过溶解的有机碳抑制微生物化物吸收和甲基化
Hyun Yoon1, Michael A P Vega1, Jiaxing Wang2
1School of Civil and Environmental Engineering, Cornell University, Ithaca, New York, 14853, United States.
概括
在大米田中的微生物甲基化被高碳基质 (如葡萄糖) 抑制. 这种碳催化剂抑制 (CCR) 机制通过 GlpF 通道影响酸盐的吸收,影响的生物地质化学.
科学领域:
- 环境微生物学 环境微生物学
- 生物地质化学生物地质化学
- 的新陈代谢过程
背景情况:
- 甲基化是循环中的关键微生物过程,特别是在大米田中.
- 微生物对化物的吸收由水糖 GlpF 介导,将其与有机碳利用联系起来.
研究的目的:
- 为了研究碳基板通过碳催化物抑制 (CCR) 抑制酸盐吸收和甲基化的假设.
- 阐明 GlpF 通道和特定碳基板在调节生物转化中的作用.
主要方法:
- 生物传感器测量剂测量的吸收.
- 用RT-qPCR分析glpF基因的表达.
- 评估酸盐甲基化由 *Arsenicibacter rosenii* 与不同的碳基板.
主要成果:
- 化物吸收被葡萄糖和溶解有机物 (DOM) 抑制.
- 增加的碳度导致*glpF*基因表达的抑制,减少了酸盐的吸收.
- 酸盐甲基化被上层的糖溶性基质 (葡萄糖,西洛斯,曼诺斯) 抑制,而不是pyruvate或succinate.
结论:
- 有机碳的可用性,特别是上层糖溶性基质,显著影响微生物甲基化.
- 碳催化剂抑制 (CCR) 是调节矿吸收和转化在田环境中的关键机制.
- 这些发现为有机碳如何影响微生物循环和生物转化提供了新的见解.
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