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整合血型定型,MLST和表型数据:解码沙门氏菌病原性和耐药性的进化驱动因素

Wanting Hong¹, Zheng Yang¹, Guoping Wu¹

¹School of Food Science & Engineering, Jiangxi Agricultural University, Nanchang, China.

Applied and environmental microbiology

|August 29, 2025

概括

全球对沙门氏菌的监测显示,抗菌素耐药性 (AMR) 和致病性正在演变. 综合基因组和表型分析强调ST34是血清型转换和耐药性的关键驱动因素,需要结合监测策略.

科学领域:

微生物学与传染病
基因组学与进化生物学
食品安全与公共卫生

背景情况:

沙门氏菌是一种具有复杂的抗菌耐药性和致病性演变的重要食源病原体.
之前的研究缺乏针对沙门氏菌的血清定型,MLST和表型数据的综合分析.
了解种群结构和进化驱动因素对于控制沙门氏菌至关重要.

研究的目的:

整合基因组学和现象学,以全面分析沙门氏菌种群结构.
调查沙门氏菌病原性和抗菌耐药性的时空动态和进化驱动因素.
确定与沙门氏菌进化和耐药性相关的关键序列类型和遗传元素.

主要方法:

来自临床和食物链来源的935个沙门氏菌分离物的分析.
综合基因组学 (血型定型,MLST) 和现象学 (抗菌耐药性分析).
时间追踪 (2018-2022) 和网络分析以确定进化集群和驱动因素.

主要成果:

沙门氏菌Typhimurium和沙门氏菌Enteritidis是主要的血清型;ST34是关键的MLST类型.
观察到对西普罗素耐药性的显著增加 (15. 3% 至 30. 4%) 和高抗四环素耐药性 (77. 3%).
ST34被确定为血清型切换的遗传骨干;S. Typhimurium显示出最高的AMR基因负担和毒性.

结论:

克隆扩张,横向基因转移和区域生态共同推动沙门氏菌的进化.
综合基因型-表型监测对于预防耐药性和毒性出现至关重要.
这些研究结果支持"One Health"的干预措施,以破坏抗药性传播并改善公共卫生结果.

关键词:

沙门氏菌抗微生物耐药性 (AMR)流行病学基因组学多部位序列类型 (MLST)

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