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Updated: Sep 9, 2025

Three-dimensional Optical-resolution Photoacoustic Microscopy

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Published on: May 3, 2011

优化的光声组合器用于高灵敏度,高速的三维光声显微镜

Ke Zhang, Erqi Tu, Xiaoyong Fang

|August 29, 2025

概括

我们开发了一种新的高速3D光学分辨率光声显微镜 (OR-PAM) 系统,

科学领域:

生物医学光学
摄影声学成像
显微镜

背景情况:

光学分辨率光声显微镜 (OR-PAM) 为生物组织成像提供了高分辨率.
传统的OR-PAM系统在声学检测灵敏度和焦点深度 (DOF) 上面临限制.

研究的目的:

开发和验证一个高速,高分辨率的3DOR-PAM系统,具有提高的灵敏度和扩展的DOF.
增强生物组织中的血管成像能力.

主要方法:

系统评估四个光声组合器 (OAC) 配置以优化声学检测灵敏度.
实施一种新的曲线扫描策略,以显著扩大DOF.
集成一个球形聚焦的超声波传感器和45°玻璃反射器,以获得最大的灵敏度.

主要成果:

优化的OAC设计实现了卓越的声学检测灵敏度.
曲面扫描策略将DOF从1.74mm扩展到大约10mm.
在扩展的 DOF 上实现了高分辨率的体积成像和均的侧面分辨率.
与平面扫描相比,体内实验表明结构可见性和血管密度有所改善.

结论:

开发的高速3DOR-PAM系统提供灵敏和大DOF的血管成像.
紧的OAC设计和光纤光传输表明手持应用的潜力.
这种平台可以对生物组织血管进行高质量的成像.

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Imaging Biological Samples with Optical Microscopy

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In optical microscopy, the specimen to be viewed is placed on a glass slide and clipped on the stage...