在表面流量构造湿地中,ARG去除有限,但抵抗力稳定
Benjamin C Davis1, David Linz1, Brian R McMinn1
1Office of Research and Development, US Environmental Protection Agency, Cincinnati, OH, 45268, USA.
Journal of environmental management
|August 29, 2025
概括
建筑处理湿地由于设计而导致抗生素耐药基因 (ARG) 的移除有限. 核心抗体保持稳定,这表明ARG是本土的,不容易转移,影响环境监测的解释.
科学领域:
- 环境科学
- 微生物学
- 水质管理
背景情况:
- 污水和排水引入微生物污染物,包括抗生素耐药细菌 (ARB) 和抗生素耐药基因 (ARG),降低水质.
- 建筑处理湿地 (CTW) 是一个低成本的流域管理解决方案,但它们对ARG的有效性需要评估.
- 了解CTW中的ARG动态对于评估它们在减轻微生物污染中的作用至关重要.
研究的目的:
- 调查ARG在修建的处理湿地中的清除效率和动态.
- 描述核心抗体,评估其稳定性和湿地生态系统中的转移潜力.
- 评估抗体结构,微生物群落和便指标生物之间的相关性.
主要方法:
- 来自Banklick Creek CTW (BCTW) 的62个水样的枪式测序以分析ARG的丰度和组成.
- 评估水力停留时间和抵抗体,微生物组和生物标志物数据之间的相关性分析.
- 研究一级整合素整合酶 (intI1) 和基因盒的含量.
主要成果:
- 观察到抗体的最小总体衰减,低丰度的ARG显示出有限的去除.
- 主要由多种药物排放系统组成的核心抗体是稳定的,并且看起来本地化,反映了区域污染.
- 抗体和微生物组结构高度相关,但ARG与移动遗传元素的同时发生是有限的,这表明转移潜力较低.
结论:
- 水平面流设计和BCTW的短停留时间限制了ARG的去除效率.
- 稳定的本地抗体表明ARG是特有的,并且在这个湿地系统中不易转移.
- 在基因卡塞特中不含ARG的intI1丰富表明需要仔细解释intI1 qPCR测定以评估ARG的移动性潜力.
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