大麻蛋糕蛋白与马尔托德克斯之间的超声强化湿加热诱导的梅拉德反应:结构特征,技术功能和抗氧化特性
Marzieh Sadeghi1, Mohammad Mousavi1, Mohammad Saeid Yarmand1
1Department of Food Science and Engineering, University of Tehran, Iran.
Ultrasonics sonochemistry
|August 29, 2025
概括
这项研究通过将其与马尔托德克斯结合来增强大麻蛋白分离物 (HPI). 湿式加热比超声波提高了功能和抗氧化性能,
科学领域:
- 食品科学与技术
- 蛋白质化学
- 生物结合
背景情况:
- 大麻蛋白分离物 (HPI) 是一种有价值的植物性蛋白质来源.
- 提高HPI的技术功能和抗氧化性能对于食品应用至关重要.
- 梅拉德反应提供了蛋白质修饰的途径.
研究的目的:
- 通过梅拉德反应将大麻蛋白分离物 (HPI) 与马尔托德克斯结合.
- 研究湿式加热和超声波辅助方法对HPI-马尔托德结合物的影响.
- 评估对结构性,功能性和抗氧化性质的影响.
主要方法:
- 使用湿热和超声波辅助的梅拉德反应进行HPI-马尔托德克斯结合.
- 通过SDS-PAGE,FTIR,XRD,光光谱和结构分析进行表征.
- 技术功能性质 (溶解性,乳化,发泡) 和抗氧化活性 (ABTS测定) 的评估.
主要成果:
- 超声波显著加快了糖化 (25. 06% DG在60分钟内),但减少了黑色素的形成.
- 湿式加热引发了更大的结构变化 (α-螺旋/β-板的减少,水性/zeta潜力的增加).
- 湿加热结合物显示出优异的溶解性 (95.77%),乳化性 (48.67% ESI),发泡性 (107.14% FC) 和抗氧化性 (71.95% ABTS).
结论:
- 湿热比超声更有效地通过糖化增强HPI的技术功能和抗氧化特性.
- 结合可显著提高HPI作为食品系统中的新成分的潜力.
- 这项研究为优化蛋白质改造提供了洞察力,
相关概念视频
Detergent Purification of Membrane Proteins
5.5K
Detergents are used to purify the integral proteins of the membrane. The hydrophobic portion of the detergent can replace membrane phospholipids while solubilizing the membrane proteins. When detergent monomers reach a specific concentration in a solution called critical micelle concentration (CMC), they form micelles. Above CMC, the concentration of the detergent monomers remains in equilibrium with the micelle. The number of detergent monomers present in the CMC varies for each detergent, and...
5.5K
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)
1.3K
When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...
1.3K
Keto–Enol Tautomerism: Mechanism
5.8K
The keto and enol forms are known as tautomers and they constantly interconvert (or tautomerize) between the two forms under acid or base catalyzed conditions. Both the reactions involve the same steps—protonation and deprotonation— although in the reverse order.
5.8K
Loss of Carboxy Group as CO2: Decarboxylation of Malonic Acid Derivatives
1.8K
Just like β-keto acids—which upon thermal decarboxylation form ketones—β-dicarboxylic acids undergo decarboxylation to generate monocarboxylic acids with the liberation of carbon dioxide.
1.8K
Masking and Demasking Agents
4.1K
EDTA titrations may necessitate masking and demasking agents to temporarily protect a particular metal ion in a mixture from the EDTA reaction. These agents facilitate the sequential analysis of the metal ions by forming stable complexes with some—but not all—metal ions during certain steps.
There are many masking agents, such as cyanide, fluoride, triethanolamine, thiourea, and 2,3-bis(sulfanyl)propan-1-ol (formerly 2,3-dimercapto-1-propanol), with the masking agent chosen based on...
There are many masking agents, such as cyanide, fluoride, triethanolamine, thiourea, and 2,3-bis(sulfanyl)propan-1-ol (formerly 2,3-dimercapto-1-propanol), with the masking agent chosen based on...
4.1K
Micelles
395
Micelle formation is an intricate process that hinges on the properties of amphiphilic or amphipathic molecules and the conditions of the system in which they are found. Amphiphilic molecules, which have both hydrophilic (water-attracting) and hydrophobic (water-repelling) parts, play a critical role in this process.In aqueous environments, these molecules arrange themselves such that their hydrophilic heads are turned towards the water phase, while their hydrophobic tails are oriented away...
395


