在受到时间热应激的牛肌细胞中转录组和蛋白组变化的表征
Erika Eckhardt1, Andrea Luttman2, Jayasimha R Daddam1
1Department of Animal Science, Michigan State University, East Lansing, MI, 48824, USA.
Journal of thermal biology
|August 29, 2025
概括
热应激显著改变牛肌细胞基因表达,增加热冲击蛋白并影响蛋白质合成途径. 极端的热应激会导致最多的分子变化,
科学领域:
- 分子生物学
- 动物科学
- 基因组学
背景情况:
- 热应激对畜牧业的生产力产生负面影响.
- 了解牛肌细胞中的分子反应对于减轻HS影响至关重要.
- 牛卫星细胞 (BSC) 是肌肉生长和修复的关键.
研究的目的:
- 在不同的热应激条件下研究牛肌细胞的分子变化.
- 对中度热应激 (MHS) 和极度热应激 (EHS) 的转录和蛋白质反应进行分析.
- 确定受热应激影响的关键基因和途径.
主要方法:
- 牛的卫星细胞分化为肌细胞.
- 肌细胞暴露在对照 (38°C),MHS (39.5°C) 或EHS (41°C) 条件下3小时.
- 对提取的RNA和蛋白质进行了转录和蛋白质分析.
主要成果:
- 在MHS和EHS中发现了显著的差异表达基因 (DEG) 和差异丰富的蛋白质 (DAP).
- 极端热应激 (EHS) 影响的基因 (2590) 比中度热应激 (MHS) (888) 更多.
- EHS上调了热冲击蛋白 (HSPs),FOXO6和PPARGC1A,影响了 anabolic 途径和肌体调节因素.
结论:
- 热应激会导致牛肌细胞的显著分子变化.
- 观察到的变化表明肌管大小和蛋白质合成的潜在表型变化.
- 准高温压力和相关途径可能为改善牛的耐热性提供策略.
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