Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Embryonic Stem Cells00:57

Embryonic Stem Cells

3.7K
Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
3.7K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Mapping and engineering the human cell-cell interactome.

Nature biotechnology·2026
Same author

Energetic gradients emerge in developing motor-microtubule structures.

bioRxiv : the preprint server for biology·2026
Same author

Decoding the origins of cellular self-organization for engineered biology.

Nature biotechnology·2026
Same author

D-SPIN constructs regulatory network models from scRNA-seq that reveal organizing principles of perturbation response.

Cell·2026
Same author

Hybrid crosses reveal a cell-type-specific landscape of mouse regulatory variation.

bioRxiv : the preprint server for biology·2026
Same author

T cell development from expanded hematopoietic progenitors reveals initiation control by <i>Lmo2</i> and Flt3L priming.

Science immunology·2026
Same journal

ARID1A terminates gastric regeneration to prevent cancer.

Developmental cell·2026
Same journal

Myc sustains sex-biased organ zonation in the Drosophila intestine.

Developmental cell·2026
Same journal

Two parallel neuronal circuits involving electrical synapse and DAF-7/TGF-β signaling regulate muscle autophagy in C. elegans.

Developmental cell·2026
Same journal

Menstruation: Once unspoken but now uncovered, one cell type at a time.

Developmental cell·2026
Same journal

The ALS- and FTD-associated proteins annexin A11 and CHMP2B act sequentially in plasma membrane repair.

Developmental cell·2026
Same journal

Organoid modeling of lung branching morphogenesis and epithelial lineage specification.

Developmental cell·2026
查看所有相关文章

相关实验视频

Updated: Sep 9, 2025

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos
12:59

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos

Published on: August 20, 2017

8.6K

用于环境研究的有效干细胞衍生小鼠胚胎模型

Victoria Jorgensen1, Min Bao1, Sergi Junyent1

  • 1Biology and Biological Engineering, California Institute of Technology, 1200 E. California Blvd, Pasadena, CA 91125, USA.

Developmental cell
|August 29, 2025
PubMed
概括
此摘要是机器生成的。

研究人员开发了一种新型的干细胞模型, 这种改进的模型有助于研究对早期胚胎形成的环境影响.

关键词:
其他国家胚胎细胞形体胚胎模型皮质细胞原始的内皮进行查皮的部分抗生素

更多相关视频

Application of Mouse Parthenogenetic Haploid Embryonic Stem Cells as a Substitute of Sperm
08:08

Application of Mouse Parthenogenetic Haploid Embryonic Stem Cells as a Substitute of Sperm

Published on: November 19, 2020

4.7K
CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models
10:57

CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models

Published on: August 24, 2022

4.1K

相关实验视频

Last Updated: Sep 9, 2025

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos
12:59

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos

Published on: August 20, 2017

8.6K
Application of Mouse Parthenogenetic Haploid Embryonic Stem Cells as a Substitute of Sperm
08:08

Application of Mouse Parthenogenetic Haploid Embryonic Stem Cells as a Substitute of Sperm

Published on: November 19, 2020

4.7K
CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models
10:57

CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models

Published on: August 24, 2022

4.1K

科学领域:

  • 发育生物学
  • 干细胞生物学
  • 遗传学

背景情况:

  • 芽细胞是干细胞衍生的芽细胞模型,对于研究早期发育至关重要.
  • 目前的布拉斯图形模型往往缺乏适当的化和原始内皮形成.

研究的目的:

  • 开发一个改进的胚胎模型来研究早期的胚胎形成.
  • 调查环境因素对布拉斯体发育的影响.

主要方法:

  • 使用胚胎干细胞 (ESCs),可诱导的GATA4-ESCs (iG4-ESCs) 和热囊细胞干细胞 (TSCs) 的模块化方法.
  • 单细胞RNA测序以分析细胞组成和与自然胚胎细胞的相似性.
  • 培养具有和没有FGF4的iG4体,以观察发育变化.

主要成果:

  • 模块化方法产生了80%效率的化胚胎状结构 (iG4-胚胎状).
  • 根据单细胞RNA测序,iG4- 体与成熟的小鼠体非常相似.
  • 没有FGF4的培养促进了壁面 trofhectoderm的特异化,12%的结构在体外显示出类似植入后的形态发生.

结论:

  • 在研究早期胚胎形成方面,iG4体是一个强大而高效的模型.
  • 这种模型系统准确地反映了咖啡因,酒精和尼古丁等环境因素对胚胎发育的影响.
  • 在研究胚胎生成和发育毒理学方面,iG4体系统提供了新的途径.