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通过CREB传感细菌膜囊泡,揭示了一个保存的宿主防御途径

Saifei Wang¹, Bohan Qi¹, Chunyu Du¹

¹Yangzhi Rehabilitation Hospital, Sunshine Rehabilitation Center, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai 20092, China.

Mucosal immunology

|August 29, 2025

概括

通过抑制CREB活性来抑制宿主免疫反应. 这种机制涉及MV中的蛋白质成分,对Drosophila和哺乳动物的肠道微生物负荷和炎症产生影响.

科学领域:

微生物学
免疫学
细胞生物学

背景情况:

细菌膜囊泡 (MVs) 介导毒性和传播,但宿主检测机制尚不清楚.
在宿主微生物相互作用中,cAMP反应元素结合蛋白 (CREB) 的作用需要进一步阐明.

研究的目的:

研究宿主如何检测细菌MV和CREB在这个过程中的作用.
描述MV对宿主免疫基因表达和信号通路的影响.

主要方法:

在MV感染后对Drosophila肠道进行转录分析.
在MV摄入后对肠细胞中的CREB活性进行分析.
微量体的分化以确定抑制成分.
在哺乳动物细胞中评估促炎因子表达.

主要成果:

来自Erwinia carotovora carotovora 15 (Ecc15) 的细菌MV诱导反应性氧物种并提高Drosophila的免疫基因.
来自Ecc15和Lactobacillus plantarum的MV通过降低Ca2+水平来抑制肠细胞中的CREB活性.
该CRTC/CREB途径抑制了PGRP-SC2依赖的氨基酶活性,独立于Imd途径影响肠道微生物负载.
通过阻断CREB活动,来自大肠杆菌的MV抑制了哺乳动物细胞中的促炎因素.

结论:

在Drosophila和哺乳动物中,CREB在检测细菌MV和启动抗感染防御方面发挥着保留作用.
细菌MV可以通过CREB抑制调节宿主免疫力,从而揭示出一种新的宿主-微生物通信途径.

关键词:

细菌膜囊泡美国 2+) 信号类植物在PCRP-SC2中

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