ADAPT-3D:用于研究和临床环境的三维光组织成像的加速深度适应处理
Daniel D Lee1,2, Kevin A Telfer3, Deanna L Davis3
1Department of Pathology & Immunology, Washington University School of Medicine, St Louis, MO, USA. ldaniel@wustl.edu.
Scientific reports
|August 29, 2025
概括
ADAPT-3D是一种新的三步方法, 这种技术保留了光和组织结构,使生物医学研究更快.
科学领域:
- 生物医学研究
- 光学成像
- 组织清除
背景情况:
- 通过光板显微镜和组织清除方法, 生物医学研究取得了进展.
- 现有的清除方案通常涉及大量的脂质去除,可能会影响细胞膜和光.
研究的目的:
- 介绍ADAPT-3D,一个简单的三步方法,用于光学组织的透明度.
- 实现跨物种的不同组织大小和类型的3D成像,同时保持结构和光.
主要方法:
- ADAPT-3D涉及部分脂质去除和无毒的水性折射率溶液.
- 该方案旨在实现速度,最小组织缩和光保护.
- 它适用于各种组织类型,包括整个小鼠大脑,头骨制剂和人类肠道组织.
主要成果:
- 在保持细胞膜和光的同时,ADAPT-3D迅速使组织变得透明.
- 在<4天的预处理后,在4小时的折射索引步骤中清除整个小鼠大脑以进行光板显微镜,而不会改变大小.
- 在24小时内清除1毫米厚的大脑切片,并在8天内清除整个小鼠头骨.
- 人类肠道组织在大约5天内被清除并免疫标记.
结论:
- ADAPT-3D为3D成像提供了高速,非收缩,光保护的工作流程.
- 这种方法弥合了基于切口和全器官研究之间的差距.
- ADAPT-3D为各种研究领域的生物发现开辟了新的途径.
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