Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Gastrulation01:56

Gastrulation

58.5K
Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata...
58.5K
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

24.4K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
24.4K
Zygotic Development And Stem Cell Formation01:10

Zygotic Development And Stem Cell Formation

5.5K
The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...
5.5K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same authorSame journal

Genome-wide profiling of histone modifications and transcription factor binding at single-cell resolution by DeChIC-seq.

Cell research·2026
Same author

Author Correction: Generation of modified cows and sheep from spermatid-like haploid embryonic stem cells.

Nature biotechnology·2026
Same author

LncRNA Gas5 directs SUV39H2 to establish heterochromatin and maintain genome stability.

Protein & cell·2026
Same author

Aberrant H3K4me3 modification of epiblast genes of extraembryonic tissue causes placental defects and implantation failure in mouse IVF embryos.

Cell reports·2026
Same author

Luteolin inhibits IL-33/ST2L-induced M2 macrophage polarization in endometriosis.

Molecular immunology·2026
Same author

Single-cell analysis reveals essential lncRNAs regulating human trophoblast lineage differentiation.

Life medicine·2026

相关实验视频

Updated: Sep 9, 2025

A Two-Step Strategy that Combines Epigenetic Modification and Biomechanical Cues to Generate Mammalian Pluripotent Cells
08:01

A Two-Step Strategy that Combines Epigenetic Modification and Biomechanical Cues to Generate Mammalian Pluripotent Cells

Published on: August 29, 2020

2.4K

通过双向多能干细胞建模胃肠后发育

Kuisheng Liu1,2, Zihui Yan1,2, Dandan Bai1,2

  • 1Shanghai Key Laboratory of Maternal Fetal Medicine, Clinical and Translational Research Center of Shanghai First Maternity and Infant Hospital, Shanghai Institute of Maternal-Fetal Medicine and Gynecologic Oncology, Frontier Science Center for Stem Cell Research, Shanghai Key Laboratory of Signaling and Disease Research, School of Life Sciences and Technology, Tongji University, Shanghai, China.

Cell research
|August 29, 2025
PubMed
概括

科学家们开发了一种新媒介来制造双向多能干细胞 (BPSC). 这些细胞有效地产生多种胚胎血统,有助于研究早期发育和创建先进的胚胎模型.

更多相关视频

A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media
07:33

A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media

Published on: August 3, 2015

8.0K
Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells
12:06

Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells

Published on: January 11, 2019

12.5K

相关实验视频

Last Updated: Sep 9, 2025

A Two-Step Strategy that Combines Epigenetic Modification and Biomechanical Cues to Generate Mammalian Pluripotent Cells
08:01

A Two-Step Strategy that Combines Epigenetic Modification and Biomechanical Cues to Generate Mammalian Pluripotent Cells

Published on: August 29, 2020

2.4K
A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media
07:33

A Novel Culture Model for Human Pluripotent Stem Cell Propagation on Gelatin in Placenta-conditioned Media

Published on: August 3, 2015

8.0K
Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells
12:06

Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells

Published on: January 11, 2019

12.5K

科学领域:

  • 发育生物学
  • 干细胞生物学
  • 遗传学

背景情况:

  • 总结早期胚胎发育是具有挑战性的,因为缺乏能够有效地产生细胞和表细胞的干细胞.
  • 现有的方法很难准确地模仿胚胎分化的初始阶段.

研究的目的:

  • 建立一种能够产生有效双向分化的干细胞的新方法.
  • 研究这些细胞在早期胚胎发育和人类细胞分化中的潜力.

主要方法:

  • 高含量的化学选,以确定最佳培养条件.
  • 建立一种用于产生小鼠双向多能干细胞 (BPSC) 的 AL介质.
  • 在体外和体内分化测试,Wnt信号通路分析和E8.5胚胎模型的生成.

主要成果:

  • 产生了与OCT4和CDX2共同表达的小鼠BPSCs,在48小时内表现出高可塑性对热囊细胞,表皮细胞和原始内皮分化.
  • 在体内,BPSC对胚胎和胚胎外组织进行了有效的贡献.
  • 通过依赖于Lef1的绕道信号过度激活被确定为关键机制.
  • 与原始内皮诱导系统的集成使得E8.5胚胎模型的高效生成能够重复关键的发育里程碑.
  • 在AL条件下培养的人类细胞显示保存的OCT4和CDX2双阳性状态.

结论:

  • 双向多能干细胞 (BPSC) 代表了研究早期血统特征的重大进展.
  • 开发的培养平台跨物种有效,为发育生物学研究提供了强大的工具.
  • 使用BPSCs生成的先进胚胎模型有助于研究胃化后的发育和形态发生.