相关实验视频
Updated: Sep 9, 2025

08:01
The Soft Agar Colony Formation Assay
Published on: October 27, 2014
112.2K
RANBP9和RANBP10在调节非小细胞肺癌的扩散方面进行合作
Arturo Orlacchio1,2, Yasuko Kajimura1,3, Lara Rizzotto4
1Department of Cancer Biology and Genetics, College of Medicine, Arthur G. James Comprehensive Cancer Center (OSUCCC), The Ohio State Universityand , Columbus, OH, USA.
Journal of experimental & clinical cancer research : CR
|August 29, 2025
概括
通过调节CTLH复合体,Scorpins (RANBP9和RANBP10) 调节非小细胞肺癌 (NSCLC) 细胞增殖. 它们的比例影响了扩散,这表明NSCLC治疗的潜在治疗点.
科学领域:
- 癌症学
- 分子生物学
- 细胞生物学
背景情况:
- 被称为Scorpins的RANBP9和RANBP10是C-终端到LisH (CTLH) 复合物的关键组成部分,这是一个不太了解的E3连接酶.
- 在非小细胞肺癌 (NSCLC) 中Scorpins的具体作用尚未被阐明.
研究的目的:
- 研究RANBP9和RANBP10在NSCLC中的独立和合作功能.
- 确定Scorpins对NSCLC细胞蛋白质和无处不在蛋白质的影响.
- 探索Scorpins作为NSCLC治疗目标的潜力.
主要方法:
- 用稳定的功能丧失和过度表达诱导细胞系来研究RANBP9和RANBP10.
- 在NSCLC患者的瘤和公共数据集中分析了RANBP9和RANBP10的表达.
- 在NSCLC细胞系中研究了与Scorpins相关的蛋白质和无处不在的变化.
主要成果:
- RANBP9和RANBP10都在NSCLC细胞中表达,并且可以独立形成功能性CTLH复合体.
- RANBP9与RANBP10的比率影响NSCLC细胞增殖,较高的比率与增殖相关.
- 过度表达RANBP10降低了NSCLC细胞增殖和与增殖相关的蛋白质水平,包括DNA复制因子.
结论:
- 斯科尔因具有抗体作用,调节CTLH复合物无处不在的输出,以调节NSCLC细胞的增殖.
- 这些发现表明,Scorpins是NSCLC生物过程的关键调节者.
- RANBP9和RANBP10是NSCLC治疗的有希望的治疗点.
关键词:
在ARMC8CTLH复合物GID复合体GID4 其他GID8 其他肺部癌症美国其他:在 NSCLC非小细胞肺癌其他国家其他国家在RANBPM在RMND5A其他国家/地区一个子时间:WDR26 时间在 YPEL5更多相关视频
相关概念视频
Abnormal Proliferation
4.6K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
The Retinoblastoma Gene
4.2K
Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.2K
Negative Regulator Molecules
35.9K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
35.9K
The Ras Gene
6.4K
The Ras-gene-encoded proteins are regulators of signaling pathways controlling cell proliferation, differentiation, or cell survival. The Ras-gene family in humans constitutes three primary members—the HRas, NRas, and KRas. These genes code for four functionally distinct yet closely related proteins—the HRas, NRas, KRas4A, and KRas4B. The involvement of mutant Ras genes in human cancer was first discovered in 1982 and is among the most common causes of human tumorigenesis.
Ras is a...
Ras is a...
6.4K
Directionality of Nuclear Transport
3.4K
Ras-related nuclear protein or Ran is a small G protein that cycles between its GTP and GDP bound states. Ran specific regulators, a Ran GTPase Activating Protein or RanGAP present in the cytosol and a Ran guanine nucleotide exchange factor or RanGEF present inside the nucleus regulate GTP/GDP exchange. A high concentration of GTP inside the cells, in addition to this asymmetric distribution of Ran-specific regulators, leads to a higher RanGTP concentration inside the nucleus. This...
3.4K
lncRNA - Long Non-coding RNAs
8.9K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.9K

