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周围和肝内B细胞的单细胞分析揭示了改变的反应和与慢性HBV感染的抗病毒潜力相关的CCR7+子组

Shihong Zhong¹, Zhaofeng Zeng¹, Zihan Jin¹

¹Department of Infectious Diseases, Nanfang Hospital, Southern Medical University, State Key Laboratory of Organ Failure Research, Key Laboratory of Infectious Diseases Research in South China (Southern Medical University), Ministry of Education, Guangdong Provincial Key Laboratory for Prevention and Control of Major Liver Diseases, Guangdong Provincial Clinical Research Center for Viral Hepatitis, Guangdong Institute of Hepatology, Guangdong Provincial Research Center for Liver Fibrosis Engineering and Technology, Guangzhou, China.

Journal of medical virology

|August 30, 2025

概括

慢性乙型肝炎病毒 (HBV) 感染的B细胞表现出功能变化,信号功能受损,抗病毒细胞因子的产生减少. 肝脏内B细胞更活跃,而CCR7+B细胞可能是有效的抗HBV治疗的关键.

科学领域:

免疫学
病毒学
细胞生物学

背景情况:

B细胞对适应性免疫和慢性乙型肝炎病毒 (HBV) 感染的结果至关重要.
对于临床应用,需要全面了解HBV感染中的B细胞反应.

研究的目的:

在慢性HBV感染中系统地描述B细胞表型,功能和转录组.
在HBV患者中比较周围和肝脏B细胞反应.

主要方法:

使用单细胞RNA测序和流细胞测量对外周和肝内B细胞进行测试.
分析了横截面和纵向患者队列.

主要成果:

慢性HBV感染的B细胞表现出功能改变,包括B细胞受体 (BCR) 信号减少和T细胞相互作用受损.
与外围B细胞相比,肝脏内B细胞的BCR信号传递,细胞因子的产生和免疫细胞的相互作用增强.
表达IL-6的CCR7+B细胞的存活率增加,并且在对治疗有反应的患者中增加.

结论:

在慢性HBV感染期间,B细胞的反应会发生动态变化.
与外围B细胞相比,肝内部B细胞具有不同的功能特征.
表达CCR7的B细胞是抗HBV策略的潜在治疗点.

关键词:

B 单元格 BCR 信号提供 CCR7+ B 细胞乙型肝炎病毒 scRNA-seq 在

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