相关实验视频

Updated: Sep 9, 2025

A Co-culture Method to Investigate the Crosstalk Between X-ray Irradiated Caco-2 Cells and PBMC

A Co-culture Method to Investigate the Crosstalk Between X-ray Irradiated Caco-2 Cells and PBMC

Published on: January 30, 2018

鲁丁增强肠道屏障功能,保护人类上皮层Caco-2细胞免受X射线辐射引起的屏障功能障碍

Ha-Young Park¹, Jin-Hee Yu^1,2, Soo-Bin Shin^1,2

¹Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup, 56212, Republic of Korea.

Molecular nutrition & food research

|August 30, 2025

概括

通过保持细胞完整性和紧密结合蛋白质, 保护肠道屏障免受辐射损伤. 这表明,在暴露在辐射中,素可能是保护肠道功能的放射性剂.

科学领域:

胃肠病学
辐射生物学
分子生物学

背景情况:

小肠上皮对于营养吸收至关重要,但容易受到辐射损伤.
目前对辐射引起的肠壁功能障碍的治疗选择有限.
聚类, 像素, 显示在保护和修复肠道屏障的潜力.

研究的目的:

研究素对肠道屏障的辐射保护作用.
确定鲁丁是否可以增强肠道上皮质的完整性,防止X射线照射.
在辐射压力下检查鲁丁对紧结蛋白表达的影响.

主要方法:

使用人类上皮层Caco-2细胞单层.
在X射线照射之前用素预处理的细胞.
评估了细胞活力,体电阻 (TEER) 和光素的透性.
密切结点标记物的量化mRNA和蛋白质表达 (ZO-2,ocludin,claudin-3).

主要成果:

常规治疗改善了Caco-2细胞的活力和屏障功能 (增加了TEER,降低了透性).
在mRNA和蛋白质水平上,常规增加了关键紧接点 (TJ) 标记物 (ZO-2,ocludin,claudin-3).
通过恢复TEER和防止TJ下调,Rutin显著减弱了X射线诱导的屏障功能.

结论:

在X射线照射下,鲁丁增强了肠道屏障的完整性和功能.
通过防止ZO-2,ocludin和claudin-3的降低调节,鲁丁可以保持上皮完整性.
鲁在辐射暴露期间具有保持肠道屏障功能的天然放射保护剂的潜力.

关键词:

卡可2细胞单层射线辐射肠道屏障保护其他紧密的结合

更多相关视频

Intestinal Epithelial Regeneration in Response to Ionizing Irradiation

Intestinal Epithelial Regeneration in Response to Ionizing Irradiation

Published on: July 27, 2022

Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis

Analyzing Beneficial Effects of Nutritional Supplements on Intestinal Epithelial Barrier Functions During Experimental Colitis

Published on: January 5, 2017

相关概念视频

Renewal of Intestinal Stem Cells

Renewal of Intestinal Stem Cells

The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Role Of Notch Signalling In Intestinal Stem Cell Renewal

Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...